Zhang, Hongtao, Palma, Angelina S., Zhang, Yibing, Childs, Robert A., Liu, Yan, Mitchell, Daniel A., Guidolin, Leticia S., Weigel, Wilfried, Mulloy, Barbara, Ciocchini, Andrés E., Feizi, Ten and Chai, Wengang (2016) Generation and characterization of β1,2-gluco-oligosaccharide probes fromBrucella abortuscyclic β-glucan and their recognition by C-type lectins of the immune system. Glycobiology, 26 (10). pp. 1086-1096. doi:10.1093/glycob/cww041 ISSN 1460-2423.

Preview

PDF WRAP_lycobiology-2016-Zhang-glycob-cww041.pdf - Published Version - Requires a PDF viewer. Download (1016Kb) | Preview

Request Changes to record.

Abstract

The β1,2-glucans produced by bacteria are important in invasion, survival and immunomodulation in infected hosts be they mammals or plants. However, there has been a lack of information on proteins which recognize these molecules. This is partly due to the extremely limited availability of the sequence-defined oligosaccharides and derived probes for use in the study of their interactions. Here we have used the cyclic β1,2-glucan (CβG) of the bacterial pathogen Brucella abortus, after removal of succinyl side chains, to prepare linearized oligosaccharides which were used to generate microarrays. We describe optimized conditions for partial depolymerization of the cyclic glucan by acid hydrolysis and conversion of the β1,2-gluco-oligosaccharides, with degrees of polymerization 2-13, to neoglycolipids for the purpose of generating microarrays. By microarray analyses we show that the C-type lectin receptor DC-SIGNR, like the closely related DC-SIGN we investigated earlier, binds to the β1,2-gluco-oligosaccharides, as does the soluble immune effector serum mannose-binding protein. Exploratory studies with DC-SIGN are suggestive of the recognition also of the intact CβG by this receptor. These findings open the way to unravelling mechanisms of immunomodulation mediated by β1,2-glucans in mammalian systems.

Item Type:	Journal Article
Subjects:	Q Science > QP Physiology
Divisions:	Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Health Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Glucans, Lectins
Journal or Publication Title:	Glycobiology
Publisher:	Oxford University Press
ISSN:	1460-2423
Official Date:	1 October 2016
Dates:	Date Event 1 October 2016 Published 18 October 2016 Available 24 March 2016 Accepted 3 February 2016 Submitted
Volume:	26
Number:	10
Page Range:	pp. 1086-1096
DOI:	10.1093/glycob/cww041
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	18 May 2016
Date of first compliant Open Access:	18 May 2016
Funder:	Wellcome Trust (London, England), Research Councils UK (RCUK), Engineering and Physical Sciences Research Council (EPSRC), Guo jia zi ran ke xue ji jin wei yuan hui (China) [National Natural Science Foundation of China] (NSFC), Fundação para a Ciência e a Tecnologia (FCT), China Scholarship Council (CSC)
Grant number:	WT093378MA (WT), WT099197MA (WT), GRS/79268 (RCUK), EP/G037604/1 (EPSRC), 31201384 (NSFC), PTDC/QUI-QUI/112537/2009 (FCT), RECI/BBB-BEP/0124/2012 (FCT), UID/Multi/04378/2013 (FCT), 2008679005 (CSC), WT099197MA (WT)

Request changes or add full text files to a record

Repository staff actions (login required)

View Item

Downloads