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The Hevea brasiliensis XIP aquaporin subfamily : genomic, structural and functional characterizations with relevance to intensive latex harvesting
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Lopez, David, Amira, Maroua Ben, Brown, Daniel, Muries, Beatriz, Brunel-Michac, Nicole, Bourgerie, Sylvain, Porcheron, Benoit, Lemoine, Remi, Chrestin, Hervé, Mollison, Ewan, Di Cola, Alessandra, Frigerio, Lorenzo, Julien, Jean-Louis, Gousset-Dupont, Aurélie, Fumanal, Boris, Label, Philippe, Pujade-Renaud, Valérie, Auguin, Daniel and Venisse, Jean-Stéphane (2016) The Hevea brasiliensis XIP aquaporin subfamily : genomic, structural and functional characterizations with relevance to intensive latex harvesting. Plant Molecular Biology, 91 (4). pp. 375-396. doi:10.1007/s11103-016-0462-y ISSN 0167-4412.
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Official URL: http://dx.doi.org/10.1007/s11103-016-0462-y
Abstract
X-Intrinsic Proteins (XIP) were recently identified in a narrow range of plants as a full clade within the aquaporins. These channels reportedly facilitate the transport of a wide range of hydrophobic solutes. The functional roles of XIP in planta remain poorly identified. In this study, we found three XIP genes (HbXIP1;1, HbXIP2;1 and HbXIP3;1) in the Hevea brasiliensis genome. Comprehensive bioinformatics, biochemical and structural analyses were used to acquire a better understanding of this AQP subfamily. Phylogenetic analysis revealed that HbXIPs clustered into two major groups, each distributed in a specific lineage of the order Malpighiales. Tissue-specific expression profiles showed that only HbXIP2;1 was expressed in all the vegetative tissues tested (leaves, stem, bark, xylem and latex), suggesting that HbXIP2;1 could take part in a wide range of cellular processes. This is particularly relevant to the rubber-producing laticiferous system, where this isoform was found to be up-regulated during tapping and ethylene treatments. Furthermore, the XIP transcriptional pattern is significantly correlated to latex production level. Structural comparison with SoPIP2;1 from Spinacia oleracea species provides new insights into the possible role of structural checkpoints by which HbXIP2;1 ensures glycerol transfer across the membrane. From these results, we discuss the physiological involvement of glycerol and HbXIP2;1 in water homeostasis and carbon stream of challenged laticifers. The characterization of HbXIP2;1 during rubber tree tapping lends new insights into molecular and physiological response processes of laticifer metabolism in the context of latex exploitation.
Item Type: | Journal Article | ||||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
Journal or Publication Title: | Plant Molecular Biology | ||||||||||
Publisher: | Springer | ||||||||||
ISSN: | 0167-4412 | ||||||||||
Official Date: | July 2016 | ||||||||||
Dates: |
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Volume: | 91 | ||||||||||
Number: | 4 | ||||||||||
Page Range: | pp. 375-396 | ||||||||||
DOI: | 10.1007/s11103-016-0462-y | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access |
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