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Transcriptional reprogramming underpins enhanced plant growth promotion by the biocontrol fungus Trichoderma hamatum GD12 during antagonistic interactions with Sclerotinia sclerotiorumin soil
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Shaw, Sophie, Le Cocq, Kate, Paszkiewicz, Konrad, Moore, Karen, Winsbury, Rebecca, de Torres Zabala, Marta, Studholme, David, Salmon, Deborah, Thornton, Christopher R. and Grant, Murray R. (2016) Transcriptional reprogramming underpins enhanced plant growth promotion by the biocontrol fungus Trichoderma hamatum GD12 during antagonistic interactions with Sclerotinia sclerotiorumin soil. Molecular Plant Pathology, 17 (9). pp. 1425-1441. doi:10.1111/mpp.12429 ISSN 1464-6722.
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WRAP_1571724-lf-240516-shaw_et_al-2016-molecular_plant_pathology.pdf - Accepted Version - Requires a PDF viewer. Download (2698Kb) | Preview |
Official URL: http://dx.doi.org/10.1111/mpp.12429
Abstract
The free-living soil fungus Trichoderma hamatum strain GD12 is notable amongst Trichoderma strains in both controlling plant diseases and in stimulating plant growth, a property enhanced during its antagonistic interactions with pathogens in soil. These attributes, alongside its markedly expanded genome and proteome compared to other biocontrol and plant growth promoting Trichoderma strains, imply a rich potential for sustainable alternatives to synthetic pesticides and fertilisers for controlling plant disease and increasing yields. The purpose of this study was to investigate the transcriptional responses of GD12 underpinning its biocontrol and plant growth promotion capabilities during antagonistic interactions with the pathogen Sclerotinia sclerotiorum in soil. Using an extensive mRNA-seq study capturing different time points during the pathogen-antagonist interaction in soil, we show that dynamic and biphasic signatures in the GD12 transcriptome underpin its biocontrol and plant (lettuce) growth promotional activities. Functional predictions of differentially expressed genes demonstrate the enrichment of transcripts encoding proteins involved in transportation and oxidation-reduction reactions during both processes and an over-representation of siderophores. We identify a biphasic response during biocontrol characterised by a significant induction of transcripts encoding small-secreted cysteine rich proteins, secondary metabolite producing gene clusters and genes unique to GD12. These data support the hypothesis that Sclerotinia biocontrol is mediated by the synthesis and secretion of antifungal compounds and that GD12's unique reservoir of uncharacterised genes is actively recruited during effective biological control of a plurivorous plant pathogen. This article is protected by copyright. All rights reserved.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QH Natural history | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
Library of Congress Subject Headings (LCSH): | Growth (Plants), Pests -- Biological control, Trichoderma | ||||||||||
Journal or Publication Title: | Molecular Plant Pathology | ||||||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||
ISSN: | 1464-6722 | ||||||||||
Official Date: | December 2016 | ||||||||||
Dates: |
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Volume: | 17 | ||||||||||
Number: | 9 | ||||||||||
Page Range: | pp. 1425-1441 | ||||||||||
DOI: | 10.1111/mpp.12429 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 25 May 2016 | ||||||||||
Date of first compliant Open Access: | 14 May 2017 | ||||||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||||
Grant number: | BB/I014691/1 (BBSRC) |
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