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Promyelocytic leukemia protein isoform II inhibits infection by human adenovirus type 5 through effects on HSP70 and the interferon response
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Atwan, Zeenah, Wright, Jordan L., Leppard, Keith and Woodman, Andrew (2016) Promyelocytic leukemia protein isoform II inhibits infection by human adenovirus type 5 through effects on HSP70 and the interferon response. Journal of General Virology, 97 . pp. 1955-1967. doi:10.1099/jgv.0.000510 ISSN 0022-1317.
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Official URL: http://dx.doi.org/10.1099/jgv.0.000510
Abstract
Promyelocytic leukemia (PML) proteins have been implicated in antiviral responses but PML and associated proteins are also suggested to support virus replication. One isoform, PML-II, is required for efficient transcription of interferon and interferon-responsive genes. . We therefore investigated the PML-II contribution to human adenovirus 5 (Ad5) infection, using shRNA-mediated knock-down. HelaΔII cells showed a 2 - 3 fold elevation in Ad5 yield, reflecting an increase in late gene expression. This increase was found to be due in part to the reduced innate immune response consequent upon PML-II depletion. However the effect was minor because the viral E4 Orf3 protein targets and inactivates this PML-II function. The major benefit to Ad5 in HelaΔII cells was exerted via an increase in HSP70; depletion of HSP70 completely reversed this replicative advantage. Increased Ad5 late gene expression was not due either to the previously described inhibition of inflammatory responses by HSP70 or to effects of HSP70 on major late promoter or L4 promoter activity but might be linked to an observed increase in E1B 55K, as this protein is known to be required for efficient late gene expression. The induction of HSP70 by PML-II removal was specific for the HSPA1B gene among the HSP70 gene family and thus was not the consequence of a general stress response. Taken together, these data show that PML-II, through its various actions, has an overall negative effect on the Ad5 life-cycle.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QR Microbiology > QR355 Virology | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Leukemia, Adenoviruses | ||||||||
| Journal or Publication Title: | Journal of General Virology | ||||||||
| Publisher: | Society for General Microbiology | ||||||||
| ISSN: | 0022-1317 | ||||||||
| Official Date: | August 2016 | ||||||||
| Dates: |
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| Volume: | 97 | ||||||||
| Page Range: | pp. 1955-1967 | ||||||||
| DOI: | 10.1099/jgv.0.000510 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Date of first compliant deposit: | 1 June 2016 | ||||||||
| Date of first compliant Open Access: | 23 May 2017 | ||||||||
| Funder: | Higher Committee for Education Development in Iraq, Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), University of Warwick |
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