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Using fluorescent reporters to monitor autophagy in the female germline cells in Drosophila melanogaster
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Jacomin, Anne-Claire and Nezis, Ioannis P. (2016) Using fluorescent reporters to monitor autophagy in the female germline cells in Drosophila melanogaster. In: Oogenesis : Methods and Protocols. Springer, pp. 69-78. ISBN 9781493937936
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Official URL: http://www.springer.com/series/7651
Abstract
Oogenesis is a fundamental biological process for the transmission of genetic information to the next generations. Drosophila has proven to be a valuable model for elucidating the molecular and cellular mechanisms involved in this developmental process. It has been shown that autophagy participates in the maturation of the egg chamber. Here we provide a protocol for monitoring and quantification of the autophagic process in the Drosophila germline cells using the fluorescent reporters mCherry-DmAtg8a and GFP-mCherry-DmAtg8a
Item Type: | Book Item | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Methods in Molecular Biology | ||||||
Publisher: | Springer | ||||||
ISBN: | 9781493937936 | ||||||
ISSN: | 1064-3745 | ||||||
Book Title: | Oogenesis : Methods and Protocols | ||||||
Official Date: | August 2016 | ||||||
Dates: |
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Volume: | 1457 | ||||||
Page Range: | pp. 69-78 | ||||||
DOI: | 10.1007/978-1-4939-3795-0_5 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Date of first compliant deposit: | 13 June 2016 | ||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||
Grant number: | BB/L006324/1 |
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