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Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis
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Zhang, Yi, Nikolovski, Nino, Sorieul, Mathias, Vellosillo, Tamara, McFarlane, Heather E., Dupree, Ray, Kesten, Christopher, Schneider, René, Driemeier, Carlos, Lathe, Rahul, Lampugnani, Edwin, Yu, Xiaolan, Ivakov, Alexander, Doblin, Monika S., Mortimer, Jenny C., Brown, Steven P., Persson, Staffan and Dupree, Paul (2016) Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis. Nature Communications, 7 . 11656. doi:10.1038/ncomms11656 ISSN 2041-1723.
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Official URL: http://dx.doi.org/10.1038/ncomms11656
Abstract
As the most abundant biopolymer on Earth, cellulose is a key structural component of the plant cell wall. Cellulose is produced at the plasma membrane by cellulose synthase (CesA) complexes (CSCs), which are assembled in the endomembrane system and trafficked to the plasma membrane. While several proteins that affect CesA activity have been identified, components that regulate CSC assembly and trafficking remain unknown. Here we show that STELLO1 and 2 are Golgi-localized proteins that can interact with CesAs and control cellulose quantity. In the absence of STELLO function, the spatial distribution within the Golgi, secretion and activity of the CSCs are impaired indicating a central role of the STELLO proteins in CSC assembly. Point mutations in the predicted catalytic domains of the STELLO proteins indicate that they are glycosyltransferases facing the Golgi lumen. Hence, we have uncovered proteins that regulate CSC assembly in the plant Golgi apparatus.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QK Botany | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Physics | ||||||||
Library of Congress Subject Headings (LCSH): | Plant cells and tissues, Biopolymers, Cellulose, Golgi apparatus | ||||||||
Journal or Publication Title: | Nature Communications | ||||||||
Publisher: | Nature Publishing Group | ||||||||
ISSN: | 2041-1723 | ||||||||
Official Date: | 9 June 2016 | ||||||||
Dates: |
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Volume: | 7 | ||||||||
Article Number: | 11656 | ||||||||
DOI: | 10.1038/ncomms11656 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 20 June 2016 | ||||||||
Date of first compliant Open Access: | 21 June 2016 | ||||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Seventh Framework Programme (European Commission) (FP7), Engineering and Physical Sciences Research Council (EPSRC), University of Warwick, Advantage West Midlands (AWM), European Regional Development Fund (ERDF), European Molecular Biology Organization (EMBO), Philomathia Foundation, Natural Sciences and Engineering Research Council of Canada (NSERC), Max-Planck-Gesellschaft zur Förderung der Wissenschaften [Max Planck Society for the Advancement of Science], University of Melbourne | ||||||||
Grant number: | BB/G016240/1 (BBSRC), FP7/2007–2013, ALTF 711–2012, ALTF-1246–2013 (EMBO), PDF-454454-2014 (NSERC) |
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