Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers

Tools
- Tools
+ Tools

Deller, Robert C., Pessin, Jeffrey E., Vatish, Manu, Mitchell, Daniel A. and Gibson, Matthew I. (2016) Enhanced non-vitreous cryopreservation of immortalized and primary cells by ice-growth inhibiting polymers. Biomaterials Science, 4 (7). pp. 1079-1084. doi:10.1039/c6bm00129g

[img]
Preview
PDF
WRAP_c6bm00129g.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution.

Download (4Mb) | Preview
Official URL: http://dx.doi.org/10.1039/C6BM00129G

Request Changes to record.

Abstract

Cell cryopreservation is an essential tool in modern biotechnology and medicine. The ability to freeze, store and distribute materials underpins basic cell biology and enables storage of donor cells needed for transplantation and regenerative medicine. However, many cell types do not survive freezing and the current state-of-the-art involves the addition of significant amounts of organic solvents as cryoprotectants, which themselves can be cytotoxic, or simply interfere with assays. A key cause of cell death in cryopreservation is ice recrystallization (growth), which primarily occurs during thawing. Here it is demonstrated that the addition of ice recrystalization inhibiting polymers to solutions containing low (non vitrifying) concentrations of DMSO enhance cell recovery rates by up to 75%. Cell functionality is also demonstrated using a placental cell line, and enhanced cryopreservation of primary rat hepatocytes is additionally shown. The crucial role of the polymers architecture (chain length) is shown, with shorter polymers being more effective than longer ones.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
R Medicine > R Medicine (General)
Divisions: Faculty of Science > Chemistry
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Cells -- Cryopreservation, Polymers, Transplantation of organs, tissues, etc., Regenerative medicine, Biotechnology, Medicine
Journal or Publication Title: Biomaterials Science
Publisher: R S C Publications
ISSN: 2047-4830
Official Date: 1 July 2016
Dates:
DateEvent
1 July 2016Published
6 May 2016Available
1 May 2016Accepted
23 February 2016Submitted
Date of first compliant deposit: 22 June 2016
Volume: 4
Number: 7
Page Range: pp. 1079-1084
DOI: 10.1039/c6bm00129g
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
Funder: Advantage West Midlands (AWM), European Regional Development Fund (ERDF), Royal Society (Great Britain), Engineering and Physical Sciences Research Council (EPSRC), University of Warwick Transatlantic Fellowship, Warwick Private Hospitals Charitable Trust, National Institutes of Health (U.S.) (NIH), European Research Council (ERC)
Grant number: EP/F500378/1 (EPSRC), DK033823 and DK020541 (NIH), CRYOMAT 638661 (ERC)
Adapted As:

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us