UNSPECIFIED. (2004) Molecular epidemiology of respiratory syncytial virus in Kilifi district, Kenya. JOURNAL OF MEDICAL VIROLOGY, 74 (2). pp. 344-354. ISSN 0146-6615

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Abstract

Respiratory syncytial virus (RSV) causes significant burden of disease during infancy and childhood. This study examined the genetic relatedness of RSV positive samples from child inpatients and outpatients and a birth cohort from a rural coastal district of Kenya and also the distribution of strains between these three groups. Clinical samples were collected over a 4-year period in Kilifi District, Kenya from community and hospital surveillance. Three hundred ninety seven of 1,044 nasal specimens from children (under 5 years old) attending Kilifi District Hospital, and from community-monitored infants, were positive for RSV by multiplex RTPCR. Of these, 376 samples were analysed further by restriction fragment length polymorphisms (RFLP) of the nucleocapsid (N) and attachment (G) protein genes. The G gene was sequenced for 109 samples and phylogenetic analysis carried out. The group A samples from Kilifi fell into two clusters based on G gene sequences, while only one group B cluster was observed. One RSV-B sample from 2003 demonstrated the presence of a 60-nucleoticle duplication within the G gene, clustering with similar isolates from Buenos Aries from 1999. All had similar sequences to isolates from the UK, USA, Spain, or Uruguay. The Kilifi District samples showed greater than 97% homology to isolates from South Africa and Mozambique and 91-94% homology to isolates from The Gambia. Samples from different sources, clearly differing in disease severity, did not differ in genotype characteristics, suggesting that disease causing variants are a general reflection of infections within this community.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title:	JOURNAL OF MEDICAL VIROLOGY
Publisher:	WILEY-LISS
ISSN:	0146-6615
Date:	October 2004
Volume:	74
Number:	2
Number of Pages:	11
Page Range:	pp. 344-354
Identification Number:	10.1002/jmv.20138
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/8074

Data sourced from Thomson Reuters' Web of Knowledge

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