The Library
2D FT-ICR MS of Calmodulin : a top-down and bottom-up approach
Tools
Floris, Federico, van Agthoven, Maria, Chiron, Lionel, Soulby, Andrew J., Wootton, Christopher, Lam, Yuko P. Y., Barrow, Mark P., Delsuc, Marc-André and O’Connor, Peter B. (2016) 2D FT-ICR MS of Calmodulin : a top-down and bottom-up approach. Journal of The American Society for Mass Spectrometry, 27 (9). pp. 1531-1538. doi:10.1007/s13361-016-1431-z ISSN 1044-0305.
PDF
WRAP_Floris_Top_down bottom_up CaM_noTracks_Final_v3.pdf - Accepted Version - Requires a PDF viewer. Download (1409Kb) |
Official URL: http://dx.doi.org/10.1007/s13361-016-1431-z
Abstract
Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry (2D FT-ICR MS) allows data-independent fragmentation of all ions in a sample and correlation of fragment ions to their precursors through the modulation of precursor ion cyclotron radii prior to fragmentation. Previous results show that implementation of 2D FT-ICR MS with infrared multi-photon dissociation (IRMPD) and electron capture dissociation (ECD) has turned this method into a useful analytical tool. In this work, IRMPD tandem mass spectrometry of calmodulin (CaM) has been performed both in one-dimensional and two-dimensional FT-ICR MS using a top-down and bottom-up approach. 2D IRMPD FT-ICR MS is used to achieve extensive inter-residue bond cleavage and assignment for CaM, using its unique features for fragment identification in a less time- and sample-consuming experiment than doing the same thing using sequential MS/MS experiments.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Library of Congress Subject Headings (LCSH): | Ion cyclotron resonance spectrometry, Calmodulin | ||||||||
Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | ||||||||
Publisher: | Springer New York LLC | ||||||||
ISSN: | 1044-0305 | ||||||||
Official Date: | September 2016 | ||||||||
Dates: |
|
||||||||
Volume: | 27 | ||||||||
Number: | 9 | ||||||||
Page Range: | pp. 1531-1538 | ||||||||
DOI: | 10.1007/s13361-016-1431-z | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 16 August 2016 | ||||||||
Date of first compliant Open Access: | 18 July 2017 | ||||||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC) | ||||||||
Grant number: | EP/J000302/1 |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year