The Library
Glycan heterogeneity on gold nanoparticles increases lectin discrimination capacity in label-free multiplexed bioassays
Tools
Otten, Lucienne, Vlachou, Denise, Richards, Sarah-Jane and Gibson, Matthew I. (2016) Glycan heterogeneity on gold nanoparticles increases lectin discrimination capacity in label-free multiplexed bioassays. Analyst, 141 (14). pp. 4305-4312. doi:10.1039/c6an00549g ISSN 0003-2654.
PDF
WRAP_Richards_c6an00549g.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (2267Kb) |
Official URL: http://dx.doi.org/10.1039/C6AN00549G
Abstract
The development of new analytical tools as point-of-care biosensors is crucial to combat the spread of infectious diseases, especially in the context of drug-resistant organisms, or to detect biological warfare agents. Glycan/lectin interactions drive a wide range of recognition and signal transduction processes within nature and are often the first site of adhesion/recognition during infection making them appealing targets for biosensors. Glycosylated gold nanoparticles have been developed that change colour from red to blue upon interaction with carbohydrate-binding proteins and may find use as biosensors, but are limited by the inherent promiscuity of some of these interactions. Here we mimic the natural heterogeneity of cell-surface glycans by displaying mixed monolayers of glycans on the surface of gold nanoparticles. These are then used in a multiplexed, label-free bioassay to create ‘barcodes’ which describe the lectin based on its binding profile. The increased information content encoded by using complex mixtures of a few sugars, rather than increased numbers of different sugars makes this approach both scalable and accessible. These nanoparticles show increased lectin identification power at a range of lectin concentrations, relative to single-channel sensors. It was also found that some information about the concentration of the lectins can be extracted, all from just a simple colour change, taking this technology closer to being a realistic biosensor.
Item Type: | Journal Article | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | R Medicine > R Medicine (General) | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||||||||
Library of Congress Subject Headings (LCSH): | Biosensors | ||||||||||
Journal or Publication Title: | Analyst | ||||||||||
Publisher: | Royal Society of Chemistry | ||||||||||
ISSN: | 0003-2654 | ||||||||||
Official Date: | 21 July 2016 | ||||||||||
Dates: |
|
||||||||||
Volume: | 141 | ||||||||||
Number: | 14 | ||||||||||
Number of Pages: | 8 | ||||||||||
Page Range: | pp. 4305-4312 | ||||||||||
DOI: | 10.1039/c6an00549g | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||
Date of first compliant deposit: | 5 August 2016 | ||||||||||
Date of first compliant Open Access: | 9 August 2016 | ||||||||||
Funder: | Advantage West Midlands (AWM), European Regional Development Fund (ERDF), Engineering and Physical Sciences Research Council (EPSRC), University of Warwick. Institute of Advanced Study (IAS), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), European Research Council (ERC) | ||||||||||
Grant number: | EP/F500378/1 (EPSRC), BB/G530233/1 (BBSRC), CRYOMAT 638661 (ERC) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year