Otten, Lucienne, Vlachou, Denise, Richards, Sarah-Jane and Gibson, Matthew I. (2016) Glycan heterogeneity on gold nanoparticles increases lectin discrimination capacity in label-free multiplexed bioassays. Analyst, 141 (14). pp. 4305-4312. doi:10.1039/c6an00549g

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Abstract

The development of new analytical tools as point-of-care biosensors is crucial to combat the spread of infectious diseases, especially in the context of drug-resistant organisms, or to detect biological warfare agents. Glycan/lectin interactions drive a wide range of recognition and signal transduction processes within nature and are often the first site of adhesion/recognition during infection making them appealing targets for biosensors. Glycosylated gold nanoparticles have been developed that change colour from red to blue upon interaction with carbohydrate-binding proteins and may find use as biosensors, but are limited by the inherent promiscuity of some of these interactions. Here we mimic the natural heterogeneity of cell-surface glycans by displaying mixed monolayers of glycans on the surface of gold nanoparticles. These are then used in a multiplexed, label-free bioassay to create ‘barcodes’ which describe the lectin based on its binding profile. The increased information content encoded by using complex mixtures of a few sugars, rather than increased numbers of different sugars makes this approach both scalable and accessible. These nanoparticles show increased lectin identification power at a range of lectin concentrations, relative to single-channel sensors. It was also found that some information about the concentration of the lectins can be extracted, all from just a simple colour change, taking this technology closer to being a realistic biosensor.

Item Type:	Journal Article
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Science > Chemistry Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Biosensors
Journal or Publication Title:	Analyst
Publisher:	Royal Society of Chemistry
ISSN:	0003-2654
Official Date:	21 July 2016
Dates:	Date Event 21 July 2016 Published 9 May 2016 Available 6 May 2016 Accepted 7 March 2016 Submitted
Date of first compliant deposit:	5 August 2016
Volume:	141
Number:	14
Number of Pages:	8
Page Range:	pp. 4305-4312
DOI:	10.1039/c6an00549g
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	Advantage West Midlands (AWM), European Regional Development Fund (ERDF), Engineering and Physical Sciences Research Council (EPSRC), University of Warwick. Institute of Advanced Study (IAS), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), European Research Council (ERC)
Grant number:	EP/F500378/1 (EPSRC), BB/G530233/1 (BBSRC), CRYOMAT 638661 (ERC)

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