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TRPA1 Channels are regulators of astrocyte basal calcium levels and long-term potentiation via constitutive D-Serine release
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Shigetomi, E., Jackson-Weaver, O., Huckstepp, Robert T. R., O'Dell, T. J. and Khakh, B. S. (2013) TRPA1 Channels are regulators of astrocyte basal calcium levels and long-term potentiation via constitutive D-Serine release. Journal of Neuroscience, 33 (24). pp. 10143-10153. doi:10.1523/JNEUROSCI.5779-12.2013 ISSN 0270-6474.
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Official URL: http://dx.doi.org/10.1523/JNEUROSCI.5779-12.2013
Abstract
Astrocytes are found throughout the brain where they make extensive contacts with neurons and synapses. Astrocytes are known to display intracellular Ca2+ signals and release signaling molecules such as D-serine into the extracellular space. However, the role(s) of astrocyte Ca2+ signals in hippocampal long-term potentiation (LTP), a form of synaptic plasticity involved in learning and memory, remains unclear. Here, we explored a recently discovered novel TRPA1 channel-mediated transmembrane Ca2+ flux pathway in astrocytes. Specifically, we determined whether block or genetic deletion of TRPA1 channels affected LTP of Schaffer collateral to CA1 pyramidal neuron synapses. Using pharmacology, TRPA1−/− mice, imaging, electrophysiology, and D-serine biosensors, our data indicate that astrocyte TRPA1 channels contribute to basal Ca2+ levels and are required for constitutive D-serine release into the extracellular space, which contributes to NMDA receptor-dependent LTP. The findings have broad relevance for the study of astrocyte–neuron interactions by demonstrating how TRPA1 channel-mediated fluxes contribute to astrocyte basal Ca2+ levels and neuronal function via constitutive D-serine release.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Journal of Neuroscience | ||||
Publisher: | Society for Neuroscience | ||||
ISSN: | 0270-6474 | ||||
Official Date: | 2013 | ||||
Dates: |
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Volume: | 33 | ||||
Number: | 24 | ||||
Page Range: | pp. 10143-10153 | ||||
DOI: | 10.1523/JNEUROSCI.5779-12.2013 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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