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An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms
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Harrison, Freya and Diggle, Stephen P. (2016) An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms. Microbiology, 162 . pp. 1755-1760. doi:10.1099/mic.0.000352 ISSN 1350-0872.
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Official URL: http://dx.doi.org/10.1099/mic.0.000352
Abstract
A key aim in microbiology is to determine the genetic and phenotypic bases of bacterial virulence, persistence and antimicrobial resistance in chronic biofilm infections. This requires tractable, high-throughput models that reflect the physical and chemical environment encountered in specific infection contexts. Such models will increase the predictive power of microbiological experiments and provide platforms for enhanced testing of novel antibacterial or antivirulence therapies. We present an optimised ex vivo model of cystic fibrosis lung infection: ex vivo culture of pig bronchiolar tissue in artificial cystic fibrosis mucus. We focus on the formation of biofilms by Pseudomonas aeruginosa. We show highly repeatable and specific formation of biofilms that resemble clinical biofilms by a commonly-studied lab strain and ten cystic fibrosis isolates of this key opportunistic pathogen.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QR Microbiology R Medicine > RC Internal medicine |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Cystic fibrosis -- Research, Pseudomonas aeruginosa, Microbiology -- Experiments, Pathogenic microorganisms | ||||||||
Journal or Publication Title: | Microbiology | ||||||||
Publisher: | Society for General Microbiology | ||||||||
ISSN: | 1350-0872 | ||||||||
Official Date: | October 2016 | ||||||||
Dates: |
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Volume: | 162 | ||||||||
Page Range: | pp. 1755-1760 | ||||||||
DOI: | 10.1099/mic.0.000352 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 19 August 2016 | ||||||||
Date of first compliant Open Access: | 11 August 2017 |
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