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A defective interfering influenza RNA inhibits infectious influenza virus replication in human respiratory tract cells : a potential new human antiviral
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Smith, Claire, Scott, Paul D., O’Callaghan, Christopher, Easton, A. J. (Andrew J.) and Dimmock, N. J. (2016) A defective interfering influenza RNA inhibits infectious influenza virus replication in human respiratory tract cells : a potential new human antiviral. Viruses, 8 (8). 237. doi:10.3390/v8080237
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Official URL: http://dx.doi.org/10.3390/v8080237
Abstract
Defective interfering (DI) viruses arise during the replication of influenza A virus and contain a non-infective version of the genome that is able to interfere with the production of infectious virus. In this study we hypothesise that a cloned DI influenza A virus RNA may prevent infection of human respiratory epithelial cells with infection by influenza A. The DI RNA (244/PR8) was derived by a natural deletion process from segment 1 of influenza A/PR/8/34 (H1N1); it comprises 395 nucleotides and is packaged in the DI virion in place of a full-length genome segment 1. Given intranasally, 244/PR8 DI virus protects mice and ferrets from clinical influenza caused by a number of different influenza A subtypes and interferes with production of infectious influenza A virus in cells in culture. However, evidence that DI influenza viruses are active in cells of the human respiratory tract is lacking. Here we show that 244/PR8 DI RNA is replicated by an influenza A challenge virus in human lung diploid fibroblasts, bronchial epithelial cells, and primary nasal basal cells, and that the yield of challenge virus is significantly reduced in a dose-dependent manner indicating that DI influenza virus has potential as a human antiviral.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QR Microbiology > QR355 Virology R Medicine > RC Internal medicine |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Influenza A virus -- Genetics, Antiviral agents, Respiratory infections, Bronchi -- Diseases, Lungs -- Diseases, Viruses -- Reproduction, RNA, Genomes, Fibroblasts, Epithelial cells | ||||||||
| Journal or Publication Title: | Viruses | ||||||||
| Publisher: | MDPI AG | ||||||||
| ISSN: | 1999-4915 | ||||||||
| Official Date: | 22 August 2016 | ||||||||
| Dates: |
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| Date of first compliant deposit: | 24 August 2016 | ||||||||
| Volume: | 8 | ||||||||
| Number: | 8 | ||||||||
| Article Number: | 237 | ||||||||
| DOI: | 10.3390/v8080237 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access | ||||||||
| RIOXX Funder/Project Grant: |
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