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Characterization of a JAZ7 activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogen Fusarium oxysporum
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Thatcher, Louise F., Cevik, Volkan, Grant, Murray R., Zhai, Bing, Jones, Jonathan D. G., Manners, John M. and Kazan, Kemal (2016) Characterization of a JAZ7 activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogen Fusarium oxysporum. Journal of Experimental Botany, 67 (8). pp. 2367-2386. doi:10.1093/jxb/erw040 ISSN 0022-0957.
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Official URL: http://dx.doi.org/10.1093/jxb/erw040
Abstract
In Arabidopsis, jasmonate (JA)-signaling plays a key role in mediating Fusarium oxysporum disease outcome. However, the roles of JASMONATE ZIM-domain (JAZ) proteins that repress JA-signaling have not been characterized in host resistance or susceptibility to this pathogen. Here, we found most JAZ genes are induced following F. oxysporum challenge, and screening T-DNA insertion lines in Arabidopsis JAZ family members identified a highly disease-susceptible JAZ7 mutant (jaz7-1D). This mutant exhibited constitutive JAZ7 expression and conferred increased JA-sensitivity, suggesting activation of JA-signaling. Unlike jaz7 loss-of-function alleles, jaz7-1D also had enhanced JA-responsive gene expression, altered development and increased susceptibility to the bacterial pathogen Pst DC3000 that also disrupts host JA-responses. We also demonstrate that JAZ7 interacts with transcription factors functioning as activators (MYC3, MYC4) or repressors (JAM1) of JA-signaling and contains a functional EAR repressor motif mediating transcriptional repression via the co-repressor TOPLESS (TPL). We propose through direct TPL recruitment, in wild-type plants JAZ7 functions as a repressor within the JA-response network and that in jaz7-1D plants, misregulated ectopic JAZ7 expression hyper-activates JA-signaling in part by disturbing finely-tuned COI1-JAZ-TPL-TF complexes.
Item Type: | Journal Article | ||||||
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Subjects: | S Agriculture > SB Plant culture | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Library of Congress Subject Headings (LCSH): | Arabidopsis, Fungal diseases of plants | ||||||
Journal or Publication Title: | Journal of Experimental Botany | ||||||
Publisher: | OUP | ||||||
ISSN: | 0022-0957 | ||||||
Official Date: | 19 February 2016 | ||||||
Dates: |
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Volume: | 67 | ||||||
Number: | 8 | ||||||
Number of Pages: | 20 | ||||||
Page Range: | pp. 2367-2386 | ||||||
DOI: | 10.1093/jxb/erw040 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 10 October 2016 | ||||||
Date of first compliant Open Access: | 11 October 2016 | ||||||
Funder: | Commonwealth Scientific and Industrial Research Organization (Australia) (CSIRO), Arabidopsis Biological Resource Center |
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