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Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection
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Casillo, Angela, Parrilli, Ermenegilda, Sannino, Filomena, Mitchell, Daniel E., Gibson, Matthew I., Marino, Gennaro, Lanzetta, Rosa, Parilli, Michelangelo, Cosconati, Sandro, Novellino, Ettore, Randazzo, Antonio, Tutino, Maria and Corsaro, Michela (2017) Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium : a strategy for cryoprotection. Carbohydrate Polymers, 156 . pp. 364-371. doi:10.1016/j.carbpol.2016.09.037
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Official URL: http://dx.doi.org/10.1016/j.carbpol.2016.09.037
Abstract
Microrganisms from sea ice, glacial and subglacial environments are currently under investigation due to their relevant ecological functions in these habitats, and to their potential biotechnological applications. The cold-adapted Colwellia psychrerythraea 34H produces extracellular polysaccharides with cryoprotection activity. We here describe the purification and detailed molecular primary and secondary structure of the exopolysaccharide (EPS) secreted by C. psychrerythraea 34H cells grown at 4°C. The structure was determined by chemical analysis and NMR. The trisaccharide repeating unit of the EPS is constituted by a N-acetyl quinovosamine unit and two residues of galacturonic acid both decorated with alanine. In addition, the EPS was tested in vitro showing a significant inhibitory effect on ice recrystallization. In-depth NMR and computational analysis suggest a pseudohelicoidal structure which seems to prevent the local tetrahedral order of the water molecules in the first hydration shell, and could be responsible of the inhibition of ice recrystallization. As cell cryopreservation is an essential tool in modern biotechnology and medicine, the observations reported in this paper could pave the way for a biotechnological application of Colwellia EPS.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history | ||||||||
| Divisions: | Faculty of Science > Chemistry | ||||||||
| Library of Congress Subject Headings (LCSH): | Cryobiology, Microorganisms, Alanine, Nuclear magnetic resonance spectroscopy, Cold adaptation | ||||||||
| Journal or Publication Title: | Carbohydrate Polymers | ||||||||
| Publisher: | Pergamon Press | ||||||||
| ISSN: | 0144-8617 | ||||||||
| Official Date: | 20 January 2017 | ||||||||
| Dates: |
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| Volume: | 156 | ||||||||
| Page Range: | pp. 364-371 | ||||||||
| DOI: | 10.1016/j.carbpol.2016.09.037 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Funder: | Royal Society (Great Britain), Engineering and Physical Sciences Research Council (EPSRC), University of Warwick. Molecular Organisation and Assembly in Cells, European Research Council (ERC) | ||||||||
| Grant number: | EP/F500378/1 (EPSRC), CRYOMAT 638661 (ERC) |
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