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Insertional mutagenesis of ricin A chain: a novel route to an anti-ricin vaccine
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UNSPECIFIED (2004) Insertional mutagenesis of ricin A chain: a novel route to an anti-ricin vaccine. VACCINE, 22 (21-22). pp. 2800-2805. doi:10.1016/j.vaccine.2004.01.024
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Official URL: http://dx.doi.org/10.1016/j.vaccine.2004.01.024
Abstract
The insertion of a specific 25-residue internal peptide into ricin toxin A chain (RTA) reduced the catalytic activity of this protein similar to300-fold. Directed proteolytic cleavage of the peptide insert essentially restored catalytic activity of the resulting two peptide A chain to normal levels. Ricin holotoxin containing unprocessed mutant A chain was not toxic to cultured mammalian cells, due to enhanced proteasomal degradation, nor was it toxic when injected into rats at a concentration that is lethal in the case of native ricin. Rats treated in this way were completely resistant to native ricin when subsequently challenged with a potentially lethal dose of the toxin. These ricin-resistant animals had a significant anti-ricin antibody titer, indicating that this approach has potential for developing an effective vaccine against this toxin. (C) 2004 Elsevier Ltd. All rights reserved.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QR Microbiology > QR180 Immunology R Medicine |
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| Journal or Publication Title: | VACCINE | ||||
| Publisher: | ELSEVIER SCI LTD | ||||
| ISSN: | 0264-410X | ||||
| Official Date: | 29 July 2004 | ||||
| Dates: |
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| Volume: | 22 | ||||
| Number: | 21-22 | ||||
| Number of Pages: | 6 | ||||
| Page Range: | pp. 2800-2805 | ||||
| DOI: | 10.1016/j.vaccine.2004.01.024 | ||||
| Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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