Insertional mutagenesis of ricin A chain: a novel route to an anti-ricin vaccine
UNSPECIFIED. (2004) Insertional mutagenesis of ricin A chain: a novel route to an anti-ricin vaccine. VACCINE, 22 (21-22). pp. 2800-2805. ISSN 0264-410XFull text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.vaccine.2004.01.024
The insertion of a specific 25-residue internal peptide into ricin toxin A chain (RTA) reduced the catalytic activity of this protein similar to300-fold. Directed proteolytic cleavage of the peptide insert essentially restored catalytic activity of the resulting two peptide A chain to normal levels. Ricin holotoxin containing unprocessed mutant A chain was not toxic to cultured mammalian cells, due to enhanced proteasomal degradation, nor was it toxic when injected into rats at a concentration that is lethal in the case of native ricin. Rats treated in this way were completely resistant to native ricin when subsequently challenged with a potentially lethal dose of the toxin. These ricin-resistant animals had a significant anti-ricin antibody titer, indicating that this approach has potential for developing an effective vaccine against this toxin. (C) 2004 Elsevier Ltd. All rights reserved.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology > QR180 Immunology
|Journal or Publication Title:||VACCINE|
|Publisher:||ELSEVIER SCI LTD|
|Date:||29 July 2004|
|Number of Pages:||6|
|Page Range:||pp. 2800-2805|
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