UNSPECIFIED. (2004) Insertional mutagenesis of ricin A chain: a novel route to an anti-ricin vaccine. VACCINE, 22 (21-22). pp. 2800-2805. ISSN 0264-410X

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Abstract

The insertion of a specific 25-residue internal peptide into ricin toxin A chain (RTA) reduced the catalytic activity of this protein similar to300-fold. Directed proteolytic cleavage of the peptide insert essentially restored catalytic activity of the resulting two peptide A chain to normal levels. Ricin holotoxin containing unprocessed mutant A chain was not toxic to cultured mammalian cells, due to enhanced proteasomal degradation, nor was it toxic when injected into rats at a concentration that is lethal in the case of native ricin. Rats treated in this way were completely resistant to native ricin when subsequently challenged with a potentially lethal dose of the toxin. These ricin-resistant animals had a significant anti-ricin antibody titer, indicating that this approach has potential for developing an effective vaccine against this toxin. (C) 2004 Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR180 Immunology R Medicine
Journal or Publication Title:	VACCINE
Publisher:	ELSEVIER SCI LTD
ISSN:	0264-410X
Official Date:	29 July 2004
Volume:	22
Number:	21-22
Number of Pages:	6
Page Range:	pp. 2800-2805
Identification Number:	10.1016/j.vaccine.2004.01.024
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/8168

Data sourced from Thomson Reuters' Web of Knowledge

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