UNSPECIFIED (2004) Gene cluster in Micromonospora echinospora ATCC15835 for the biosynthesis of the gentamicin C complex. JOURNAL OF ANTIBIOTICS, 57 (7). pp. 436-445. ISSN 0021-8820

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Abstract

Gentamicin is a 4,6-disubstituted aminocyclitol antibiotic complex synthesised by some members of the actinomycete genus Micromonospora. In a search for the gentamicin biosynthetic gene cluster we identified, using a cosmid library approach, a region of the M. echinospora ATCC15835 chromosome that encodes homologues of aminoglycoside biosynthesis genes including gntB-a close homologue of the 2-deoxy-scyllo-inosose synthase gene (btrC) from butirosin-producing Bacillus circulans. Insertional inactivation was achieved by homologous recombination with an internal gntB fragment-containing suicide plasmid, delivered by conjugal transfer from Escherichia coli. gntB disruptants were gentamicin non-producing mutants as assayed by an ELISA antibiotic detection system, proving the association of gntB (or a downstream region) with gentamicin biosynthesis. The function of some open reading frames within the cluster, predicted by nucleotide database homology searching, is discussed with regards to their potential roles in gentamicin biosynthesis. The discovery of this genetic region represents the first report of a gene cluster involved in the biosynthesis of a 4,6-disubstituted aminocyclitol antibiotic.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology Q Science > QR Microbiology > QR180 Immunology Q Science > QR Microbiology R Medicine > RS Pharmacy and materia medica
Journal or Publication Title:	JOURNAL OF ANTIBIOTICS
Publisher:	JAPAN ANTIBIOT RES ASSN
ISSN:	0021-8820
Date:	July 2004
Volume:	57
Number:	7
Number of Pages:	10
Page Range:	pp. 436-445
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/8178

Data sourced from Thomson Reuters' Web of Knowledge

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