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Expression of 25-hydroxyvitamin D-3-1 alpha-hydroxylase in pancreatic islets
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UNSPECIFIED. (2004) Expression of 25-hydroxyvitamin D-3-1 alpha-hydroxylase in pancreatic islets. Journal of Molecular Biology, 89-90 (1-5 Sp. Iss. SI). pp. 121-125. ISSN 0960-0760
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Official URL: http://dx.doi.org/10.1016/j.jsbmb.2004.03.115
Abstract
A number of studies have suggested that Vitamin D has a potential role in the development/treatment of diabetes. These effects may be mediated by circulating levels of I alpha,25(OH)(2)D-3, but local production of 1alpha,25(OH)2D3, catalysed by the enzyme 25-hydroxyvitamin D-3-1alpha-hydroxylase (1alpha-OHase), is also likely to be important. RT-PCR analyses demonstrated that both isolated rat islets and MIN6 cells (mouse insulin-secreting cell line, characteristic of P cells) expressed 1alpha-OHase mRNA. The transcript in both cell types was similar to that seen in HKC-8 cells (a renal cell line, which expresses 1alpha-OHase). Western blot analysis and immunolocalisation identified I alpha-OHase protein in MIN6 cells and human pancreatic tissue. In addition, suspensions of rat islets were able to convert [H-3]-25-hydroxyvitamin D-3 to [H-3]-1alpha,25(OH)(2)D-3, demonstrating 1alpha-OHase activity. Both cell systems expressed the Vitamin D receptor and 1alpha,25(OH)(2)D-3 (50 nM) evoked a rapid rise in [Ca2+], in MIN6 cells. This data clearly demonstrates islets are able to produce 1alpha,25(OH)(2)D-3 and respond rapidly to treatment with 1alpha,25(OH)(2)D-3. Therefore, we would postulate that local production of 1alpha,25(OH)(2)D-3 maybe an important autocrine link between Vitamin D status and pancreatic function. (C) 2004 Elsevier Ltd. All rights reserved.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QD Chemistry R Medicine > RC Internal medicine |
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| Journal or Publication Title: | Journal of Molecular Biology | ||||
| Publisher: | PERGAMON-ELSEVIER SCIENCE LTD | ||||
| ISSN: | 0960-0760 | ||||
| Official Date: | May 2004 | ||||
| Dates: |
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| Volume: | 89-90 | ||||
| Number: | 1-5 Sp. Iss. SI | ||||
| Number of Pages: | 5 | ||||
| Page Range: | pp. 121-125 | ||||
| Identification Number: | 10.1016/j.jsbmb.2004.03.115 | ||||
| Publication Status: | Published | ||||
| URI: | http://wrap.warwick.ac.uk/id/eprint/8198 |
Data sourced from Thomson Reuters' Web of Knowledge
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