Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Expression of 25-hydroxyvitamin D-3-1 alpha-hydroxylase in pancreatic islets

Tools
- Tools
+ Tools

UNSPECIFIED (2004) Expression of 25-hydroxyvitamin D-3-1 alpha-hydroxylase in pancreatic islets. Journal of Molecular Biology, 89-90 (1-5 Sp. Iss. SI). pp. 121-125. doi:10.1016/j.jsbmb.2004.03.115

Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1016/j.jsbmb.2004.03.115

Request Changes to record.

Abstract

A number of studies have suggested that Vitamin D has a potential role in the development/treatment of diabetes. These effects may be mediated by circulating levels of I alpha,25(OH)(2)D-3, but local production of 1alpha,25(OH)2D3, catalysed by the enzyme 25-hydroxyvitamin D-3-1alpha-hydroxylase (1alpha-OHase), is also likely to be important. RT-PCR analyses demonstrated that both isolated rat islets and MIN6 cells (mouse insulin-secreting cell line, characteristic of P cells) expressed 1alpha-OHase mRNA. The transcript in both cell types was similar to that seen in HKC-8 cells (a renal cell line, which expresses 1alpha-OHase). Western blot analysis and immunolocalisation identified I alpha-OHase protein in MIN6 cells and human pancreatic tissue. In addition, suspensions of rat islets were able to convert [H-3]-25-hydroxyvitamin D-3 to [H-3]-1alpha,25(OH)(2)D-3, demonstrating 1alpha-OHase activity. Both cell systems expressed the Vitamin D receptor and 1alpha,25(OH)(2)D-3 (50 nM) evoked a rapid rise in [Ca2+], in MIN6 cells. This data clearly demonstrates islets are able to produce 1alpha,25(OH)(2)D-3 and respond rapidly to treatment with 1alpha,25(OH)(2)D-3. Therefore, we would postulate that local production of 1alpha,25(OH)(2)D-3 maybe an important autocrine link between Vitamin D status and pancreatic function. (C) 2004 Elsevier Ltd. All rights reserved.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
R Medicine > RC Internal medicine
Journal or Publication Title: Journal of Molecular Biology
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
ISSN: 0960-0760
Official Date: May 2004
Dates:
DateEvent
May 2004UNSPECIFIED
Volume: 89-90
Number: 1-5 Sp. Iss. SI
Number of Pages: 5
Page Range: pp. 121-125
DOI: 10.1016/j.jsbmb.2004.03.115
Publication Status: Published

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us