Expression of 25-hydroxyvitamin D-3-1 alpha-hydroxylase in pancreatic islets
UNSPECIFIED. (2004) Expression of 25-hydroxyvitamin D-3-1 alpha-hydroxylase in pancreatic islets. Journal of Molecular Biology, 89-90 (1-5 Sp. Iss. SI). pp. 121-125. ISSN 0960-0760Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.jsbmb.2004.03.115
A number of studies have suggested that Vitamin D has a potential role in the development/treatment of diabetes. These effects may be mediated by circulating levels of I alpha,25(OH)(2)D-3, but local production of 1alpha,25(OH)2D3, catalysed by the enzyme 25-hydroxyvitamin D-3-1alpha-hydroxylase (1alpha-OHase), is also likely to be important. RT-PCR analyses demonstrated that both isolated rat islets and MIN6 cells (mouse insulin-secreting cell line, characteristic of P cells) expressed 1alpha-OHase mRNA. The transcript in both cell types was similar to that seen in HKC-8 cells (a renal cell line, which expresses 1alpha-OHase). Western blot analysis and immunolocalisation identified I alpha-OHase protein in MIN6 cells and human pancreatic tissue. In addition, suspensions of rat islets were able to convert [H-3]-25-hydroxyvitamin D-3 to [H-3]-1alpha,25(OH)(2)D-3, demonstrating 1alpha-OHase activity. Both cell systems expressed the Vitamin D receptor and 1alpha,25(OH)(2)D-3 (50 nM) evoked a rapid rise in [Ca2+], in MIN6 cells. This data clearly demonstrates islets are able to produce 1alpha,25(OH)(2)D-3 and respond rapidly to treatment with 1alpha,25(OH)(2)D-3. Therefore, we would postulate that local production of 1alpha,25(OH)(2)D-3 maybe an important autocrine link between Vitamin D status and pancreatic function. (C) 2004 Elsevier Ltd. All rights reserved.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry
R Medicine > RC Internal medicine
|Journal or Publication Title:||Journal of Molecular Biology|
|Publisher:||PERGAMON-ELSEVIER SCIENCE LTD|
|Official Date:||May 2004|
|Number:||1-5 Sp. Iss. SI|
|Number of Pages:||5|
|Page Range:||pp. 121-125|
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