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Genetic diversity of Campylobacter jejuni and Campylobacter coli isolates from conventional broiler flocks and the impacts of sampling strategy and laboratory method
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Vidal, A. B., Colles, F. M., Rodgers, J. D., McCarthy, Noel D., Davies, R. H., Maiden, M. C. J. and Clifton-Hadley, F. A. (2016) Genetic diversity of Campylobacter jejuni and Campylobacter coli isolates from conventional broiler flocks and the impacts of sampling strategy and laboratory method. Applied and Environmental Microbiology, 82 (8). pp. 2347-2355. doi:10.1128/AEM.03693-15 ISSN 0099-2240.
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Official URL: http://dx.doi.org/10.1128/AEM.03693-15
Abstract
The genetic diversity of Campylobacter jejuni and Campylobacter coli isolates from commercial broiler farms was examined by multilocus sequence typing (MLST), with an assessment of the impact of the sample type and laboratory method on the genotypes of Campylobacter isolated. A total of 645 C. jejuni and 106 C. coli isolates were obtained from 32 flocks and 17 farms, with 47 sequence types (STs) identified. The Campylobacter jejuni isolates obtained by different sampling approaches and laboratory methods were very similar, with the same STs identified at similar frequencies, and had no major effect on the genetic profile of Campylobacter population in broiler flocks at the farm level. For C. coli, the results were more equivocal. While some STs were widely distributed within and among farms and flocks, analysis of molecular variance (AMOVA) revealed a high degree of genetic diversity among farms for C. jejuni, where farm effects accounted for 70.5% of variance, and among flocks from the same farm (9.9% of variance for C. jejuni and 64.1% for C. coli). These results show the complexity of the population structure of Campylobacter in broiler production and that commercial broiler farms provide an ecological niche for a wide diversity of genotypes. The genetic diversity of C. jejuni isolates among broiler farms should be taken into account when designing studies to understand Campylobacter populations in broiler production and the impact of interventions. We provide evidence that supports synthesis of studies on C. jejuni populations even when laboratory and sampling methods are not identical.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology S Agriculture > SF Animal culture |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Health Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Health Sciences > Population, Evidence & Technologies (PET) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Campylobacter jejuni, Campylobacter infections in poultry, Food poisoning, Broilers (Chickens) | ||||
Journal or Publication Title: | Applied and Environmental Microbiology | ||||
Publisher: | American Society for Microbiology | ||||
ISSN: | 0099-2240 | ||||
Official Date: | 12 February 2016 | ||||
Dates: |
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Volume: | 82 | ||||
Number: | 8 | ||||
Page Range: | pp. 2347-2355 | ||||
DOI: | 10.1128/AEM.03693-15 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 11 October 2016 | ||||
Date of first compliant Open Access: | 11 October 2016 | ||||
Funder: | Great Britain. Department for Environment, Food and Rural Affairs (DEFRA) | ||||
Grant number: | Grant number OZ0615 |
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