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Spatial and temporal variability in the potential of river water biofilms to degrade p-nitrophenol
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Kowalczyk, Agnieszka, Price, Oliver R., van der Gast, Christopher J., Finnegan, Christopher J., van Egmond, Roger A., Schäfer, Hendrik and Bending, G. D. (2016) Spatial and temporal variability in the potential of river water biofilms to degrade p-nitrophenol. Chemosphere, 164 . pp. 355-362. doi:10.1016/j.chemosphere.2016.08.095 ISSN 0045-6535.
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Official URL: http://dx.doi.org/10.1016/j.chemosphere.2016.08.09...
Abstract
In order to predict the fate of chemicals in the environment, a range of regulatory tests are performed with microbial inocula collected from environmental compartments to investigate the potential for biodegradation. The abundance and distribution of microbes in the environment is affected by a range of variables, hence diversity and biomass of inocula used in biodegradation tests can be highly variable in space and time. The use of artificial or natural biofilms in regulatory tests could enable more consistent microbial communities be used as inocula, in order to increase test consistency. We investigated spatial and temporal variation in composition, biomass and chemical biodegradation potential of bacterial biofilms formed in river water. Sampling time and sampling location impacted the capacity of biofilms to degrade p-nitrophenol (PNP). Biofilm bacterial community structure varied across sampling times, but was not affected by sampling location. Degradation of PNP was associated with increased relative abundance of Pseudomonas syringae. Partitioning of the bacterial metacommunity into core and satellite taxa revealed that the P. syringae could be either a satellite or core member of the community across sampling times, but this had no impact on PNP degradation. Quantitative PCR analysis of the pnpA gene showed that it was present in all samples irrespective of their ability to degrade PNP. River biofilms showed seasonal variation in biomass, microbial community composition and PNP biodegradation potential, which resulted in inconsistent biodegradation test results. We discuss the results in the context of the mechanisms underlying variation in regulatory chemical degradation tests.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
Library of Congress Subject Headings (LCSH): | Biofilms -- Biodegradation, Pseudomonas, Stream chemistry | ||||||||||
Journal or Publication Title: | Chemosphere | ||||||||||
Publisher: | Pergamon | ||||||||||
ISSN: | 0045-6535 | ||||||||||
Official Date: | December 2016 | ||||||||||
Dates: |
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Volume: | 164 | ||||||||||
Page Range: | pp. 355-362 | ||||||||||
DOI: | 10.1016/j.chemosphere.2016.08.095 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||
Date of first compliant deposit: | 17 October 2016 | ||||||||||
Date of first compliant Open Access: | 3 September 2017 | ||||||||||
Funder: | Unilever (Firm). Safety and Environmental Assurance Centre |
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