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Altered CO2 sensitivity of connexin26 mutant hemichannels in vitro
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de Wolf, Elizabeth, van de Wiel, Joseph, Cook, Jonathan and Dale, Nicholas (2016) Altered CO2 sensitivity of connexin26 mutant hemichannels in vitro. Physiological Reports, 4 (22). e13038. ISSN 2051-817X.
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Official URL: https://doi.org/10.14814/phy2.13038
Abstract
Connexin26 (Cx26) mutations underlie human pathologies ranging from hearing loss to keratitis ichthyosis deafness (KID) syndrome. Cx26 hemichannels are directly gated by CO2 and contribute to the chemosensory regulation of breathing. The KID syndrome mutation A88V is insensitive to CO2, and has a dominant negative action on the CO2 sensitivity of Cx26WT hemichannels, and reduces respiratory drive in humans. We have now examined the effect of further human mutations of Cx26 on its sensitivity to CO2. Mutated Cx26 subunits, carrying one of A88S, N14K, N14Y, M34T, or V84L, were transiently expressed in HeLa cells. The CO2‐dependence of hemichannel activity, and their ability to exert dominant negative actions on cells stably expressing Cx26WT, was quantified by a dye‐loading assay. The KID syndrome mutation, N14K, abolished the sensitivity of Cx26 to CO2. Both N14Y and N14K exerted a powerful dominant negative action on the CO2 sensitivity of Cx26WT. None of the other mutations (all recessive) had a dominant negative action. A88S shifted the affinity of Cx26 to slightly higher levels without reducing its ability to fully open to CO2. M34T did not change the affinity of Cx26 for CO2 but reduced its ability to open in response to CO2. V84L had no effect on the CO2‐sensitivity of Cx26. Some pathological mutations of Cx26 can therefore alter the CO2 sensitivity of Cx26 hemichannels. The loss of CO2 sensitivity could contribute to pathology and consequent reduced respiratory drive could be an unrecognized comorbidity of these pathologies.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QH Natural history | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Library of Congress Subject Headings (LCSH): | Connexins, Carbon dioxide | ||||||
Journal or Publication Title: | Physiological Reports | ||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||
ISSN: | 2051-817X | ||||||
Official Date: | 24 November 2016 | ||||||
Dates: |
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Volume: | 4 | ||||||
Number: | 22 | ||||||
Article Number: | e13038 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 31 October 2016 | ||||||
Date of first compliant Open Access: | 23 May 2017 | ||||||
Funder: | Leverhulme Trust (LT) | ||||||
Grant number: | RPG-2015-090 | ||||||
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