The Library
cis-cinnamic acid is a novel, natural auxin efflux inhibitor that promotes lateral root formation
Tools
Tools
Tools
Steenackers, Ward Jan, Klíma, Petr, Quareshy, Mussa, Cesarino, Igor, Kumpf, Robert P., Corneillie, Sander, Araújo, Pedro, Viaene, Tom, Goeminne, Geert, Nowack, Moritz K, Ljung, Karin, Friml, Jiří, Blakeslee, Joshua J, Novák, Ondřej, Zažímalová, Eva, Napier, R. (Richard), Boerjan, Wout A and Vanholme, Bartel (2016) cis-cinnamic acid is a novel, natural auxin efflux inhibitor that promotes lateral root formation. Plant Physiology, 173 (1). pp. 552-565. doi:10.1104/pp.16.00943 ISSN 0032-0889.
![[img]](http://wrap.warwick.ac.uk/style/images/fileicons/application_pdf.png) |
PDF
WRAP_0380721-lf-151116-ca_paper_from_plant_phys.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (3895Kb) |
Official URL: http://dx.doi.org/10.1104/pp.16.00943
Abstract
Auxin steers numerous physiological processes in plants making the tight control of its endogenous levels and spatiotemporal distribution a necessity. This regulation is achieved by different mechanisms including auxin biosynthesis, metabolic conversions, degradation and transport. Here we introduce cis-cinnamic acid (c-CA) as a novel and unique addition to a small group of endogenous molecules affecting in planta auxin concentrations. c-CA is the photo-isomerization product of the phenylpropanoid pathway intermediate trans-CA (t-CA). When grown on c-CA-containing medium, an evolutionary diverse set of plant species where shown to exhibit phenotypes characteristic for high auxin levels, including inhibition of primary root growth, induction of root hairs, and promotion of adventitious and lateral rooting. By molecular docking and receptor binding assays, we showed that c-CA itself is neither an auxin, nor an anti-auxin, and auxin profiling data revealed that c-CA does not significantly interfere with auxin biosynthesis. Single-cell-based auxin accumulation assays showed that c-CA, and not t-CA, is a potent inhibitor of auxin efflux. Auxin signaling reporters detected changes in spatiotemporal distribution of the auxin response along the root of c-CA-treated plants, and long distance auxin transport assays showed no inhibition of rootward auxin transport. Overall, these results suggest that the phenotypes of c-CA-treated plants are the consequence of a local change in auxin accumulation, induced by the inhibition of auxin efflux transport. This work reveals a novel mechanism how plants may regulate auxin levels and adds a novel, naturally occurring molecule to the chemical toolbox for the studies of auxin homeostasis.
| Item Type: | Journal Article | ||||||
|---|---|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
| Journal or Publication Title: | Plant Physiology | ||||||
| Publisher: | American Society of Plant Biologists | ||||||
| ISSN: | 0032-0889 | ||||||
| Official Date: | 11 November 2016 | ||||||
| Dates: |
|
||||||
| Volume: | 173 | ||||||
| Number: | 1 | ||||||
| Page Range: | pp. 552-565 | ||||||
| DOI: | 10.1104/pp.16.00943 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
| Date of first compliant deposit: | 17 November 2016 |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
