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Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanism
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Britto, Mishan, Goulet, Adeline, Rizvi, Syeda, von Loeffelholz, Ottilie, Moores, Carolyn A. and Cross, R. A. (2016) Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanism. Proceedings of the National Academy of Sciences of the United States of America, 113 (47). E7483-E7489. doi:10.1073/pnas.1611581113 ISSN 0027-8424.
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Official URL: http://dx.doi.org/10.1073/pnas.1611581113
Abstract
Molecular motors organize cells by hauling molecular cargoes along polymer tracks (actin filaments or microtubules). Until recently, the stepping direction of each motor was thought to be fixed; however, it now emerges that yeast kinesin-5 motors can reverse their stepping direction. How does this work? We show that the stepping direction of Cut7, a yeast kinesin-5 motor, depends on the level of motor crowding on the microtubule, and that crowding of Cut7 by non-Cut7 proteins also can drive reversal. To explain this, we propose that stepping of Cut7 in one direction is blocked by collisions with neighbors, whereas stepping in the other direction, being less space-hungry, is not. Crowding-dependent directional reversal is a hitherto-unsuspected aspect of motor-driven self-organization in cells.
| Item Type: | Journal Article | ||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Proceedings of the National Academy of Sciences of the United States of America | ||||||||
| Publisher: | National Academy of Sciences | ||||||||
| ISSN: | 0027-8424 | ||||||||
| Official Date: | 22 November 2016 | ||||||||
| Dates: |
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| Volume: | 113 | ||||||||
| Number: | 47 | ||||||||
| Page Range: | E7483-E7489 | ||||||||
| DOI: | 10.1073/pnas.1611581113 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 18 October 2018 | ||||||||
| Date of first compliant Open Access: | 18 October 2018 |
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