Zhu, Z-X., Yu, Z-M., Taylor, Jemma L., Wu, Y-H. and Ni, J. (2016) The application of yeast hybrid systems in protein interaction analysis. Molecular Biology, 50 (5). pp. 663-670. doi:10.7868/S0026898416050189

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Abstract

Yeast hybrid systems have been widely used due to their convenience and low cost. Based on these systems, many methods have been developed to analyze protein-protein, protein-DNA and protein-RNA interactions. In this paper, we are reviewing these different yeast hybrid systems. According to the number of hybrid proteins, yeast hybrid systems can be divided into three categories, yeast one-hybrid, yeast two-hybrid and yeast three-hybrid systems. Alternatively, yeast hybrid systems can be categorized according to the subcellular localization of the protein interaction process in the cell into nuclear protein-protein interactions, cytosol protein-protein interactions and membrane protein-protein interactions. Throughout the review, we focus on the progress and limitations of each yeast hybrid system over the recent years.

Item Type:	Journal Article
Subjects:	?? GAL4 ?? ?? cytosol ?? ?? interaction ?? ?? membrane ?? ?? nuclear ?? ?? protein ?? ?? yeast hybrid system ??
Divisions:	Faculty of Science > Life Sciences (2010- )
SWORD Depositor:	Library Publications Router
Journal or Publication Title:	Molecular Biology
Publisher:	Springer
ISSN:	0026-8984
Official Date:	September 2016
Dates:	Date Event September 2016 Published 19 October 2016 Available
Volume:	50
Number:	5
Page Range:	pp. 663-670
DOI:	10.7868/S0026898416050189
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Version or Related Resource:	Published in Russian in Molekulyarnaya Biologiya, 2016, Vol. 50, No. 5, pp. 751–759.

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