The Library
Quality of placental RNA : effects of explant size and culture duration
Tools
Brew, O., Nikolopoulou, E., Hughes, A., Christian, Mark, Lee, Y., Oduwole, O., Sullivan, M. H. F. and Woodman, Andrew (2016) Quality of placental RNA : effects of explant size and culture duration. Placenta, 46 . pp. 45-48. doi:10.1016/j.placenta.2016.08.083 ISSN 0143-4004.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1016/j.placenta.2016.08.083
Abstract
We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||||||
Journal or Publication Title: | Placenta | ||||||||
Publisher: | Elsevier Ltd. | ||||||||
ISSN: | 0143-4004 | ||||||||
Official Date: | October 2016 | ||||||||
Dates: |
|
||||||||
Volume: | 46 | ||||||||
Page Range: | pp. 45-48 | ||||||||
DOI: | 10.1016/j.placenta.2016.08.083 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Adapted As: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |