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Membrane protein extraction and purification using styrene-maleic acid (SMA) co-polymer: effect of variations in polymer structure
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Morrison, K. A., Akram, A., Mathews, A., Khan, Z. A., Patel, J. H., Zhou, C., Hardy, D. J., Moore-Kelly, C., Patel, R., Odiba, V., Knowles, T., Javed, M.-u.-H., Chmel, Nikola Paul, Dafforn, T. R. and Rothnie, A. J. (2016) Membrane protein extraction and purification using styrene-maleic acid (SMA) co-polymer: effect of variations in polymer structure. Biochemical Journal, 473 (23). pp. 4349-4360. doi:10.1042/BCJ20160723 ISSN 0264-6021.
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Official URL: http://dx.doi.org/10.1042/BCJ20160723
Abstract
The use of styrene–maleic acid (SMA) copolymers to extract and purify transmembrane proteins, while retaining their native bilayer environment, overcomes many of the disadvantages associated with conventional detergent-based procedures. This approach has huge potential for the future of membrane protein structural and functional studies. In this investigation, we have systematically tested a range of commercially available SMA polymers, varying in both the ratio of styrene and maleic acid and in total size, for the ability to extract, purify and stabilise transmembrane proteins. Three different membrane proteins (BmrA, LeuT and ZipA), which vary in size and shape, were used. Our results show that several polymers, can be used to extract membrane proteins, comparably to conventional detergents. A styrene:maleic acid ratio of either 2:1 or 3:1, combined with a relatively small average molecular mass (7.5–10 kDa), is optimal for membrane extraction, and this appears to be independent of the protein size, shape or expression system. A subset of polymers were taken forward for purification, functional and stability tests. Following a one-step affinity purification, SMA 2000 was found to be the best choice for yield, purity and function. However, the other polymers offer subtle differences in size and sensitivity to divalent cations that may be useful for a variety of downstream applications.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
| Journal or Publication Title: | Biochemical Journal | ||||||||
| Publisher: | Portland Press | ||||||||
| ISSN: | 0264-6021 | ||||||||
| Official Date: | 25 November 2016 | ||||||||
| Dates: |
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| Volume: | 473 | ||||||||
| Number: | 23 | ||||||||
| Page Range: | pp. 4349-4360 | ||||||||
| DOI: | 10.1042/BCJ20160723 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access |
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