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Comparison of RAFT derived poly(vinylpyrolidone) verses Poly(oligoethyleneglycol methacrylate) for the stabilization of glycosylated gold nanoparticles
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Ieong , Nga Sze, Biggs, Caroline I., Walker , Mark and Gibson, Matthew I. (2017) Comparison of RAFT derived poly(vinylpyrolidone) verses Poly(oligoethyleneglycol methacrylate) for the stabilization of glycosylated gold nanoparticles. Journal of Polymer Science Part A : Polymer Chemistry, 55 (7). pp. 1200-1280. doi:10.1002/pola.28481 ISSN 1099-0518.
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Official URL: http://doi.org/10.1002/pola.28481
Abstract
Carbohydrates dictate many biological processes including infection by pathogens. Glycosylated polymers and nanomaterials which have increased affinity due to the cluster glycoside effect, are therefore useful tools to probe function, but also as prophylactic therapies or diagnostic tools. Here, the effect of polymer structure on the coating of gold nanoparticles is studied in the context of grafting density, buffer stability and in a lectin binding assay. RAFT polymerization is used to generate poly(oligoethyleneglycol methacrylates) and poly(N-vinyl pyrolidones) with a thiol end-group for subsequent immobilization onto the gold. It is observed that poly(oligoethylene glycol methacrylates), despite being widely used particle coatings, lead to low grafting densities which in turn resulted in lower stability in biological buffers. A depression of the cloud point upon nanoparticle immobilization is also seen, which might compromise performance. In comparison poly(vinyl pyrolidones) resulted in stable particles with higher grafting densities due to the compact size of each monomer unit. The higher grafting density also enabled an increase in the number of carbohydrates which can be installed per nanoparticle at the chain ends, and gave increased binding in a lectin recognition assay. These results will guide the development of new nanoparticle biosensors with enhanced specificity, affinity and stability
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QD Chemistry R Medicine > RS Pharmacy and materia medica |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||
Library of Congress Subject Headings (LCSH): | Nanoparticles, Polymers, Gold, Polymerization, Glycosylation, Fragmentation reactions | ||||||||||
Journal or Publication Title: | Journal of Polymer Science Part A : Polymer Chemistry | ||||||||||
Publisher: | Wiley | ||||||||||
ISSN: | 1099-0518 | ||||||||||
Official Date: | 1 April 2017 | ||||||||||
Dates: |
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Volume: | 55 | ||||||||||
Number: | 7 | ||||||||||
Page Range: | pp. 1200-1280 | ||||||||||
DOI: | 10.1002/pola.28481 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 7 December 2016 | ||||||||||
Date of first compliant Open Access: | 26 January 2018 | ||||||||||
Funder: | Birmingham Science City, Advantage West Midlands (AWM), European Regional Development Fund (ERDF), European Research Council (ERC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||||
Grant number: | CRYOMAT 638661 (ERC) |
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