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Non-redundant contribution of the plastidial FAD8 ω-3 desaturase to glycerolipid unsaturation at different temperatures in arabidopsis
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Román, Ángela, Hernández, María L., Soria-García, Ángel, López-Gomollón, Sara, Lagunas, Beatriz, Picorel, Rafael, Martínez-Rivas, José Manuel and Alfonso, Miguel (2015) Non-redundant contribution of the plastidial FAD8 ω-3 desaturase to glycerolipid unsaturation at different temperatures in arabidopsis. Molecular Plant - Microbe Interactions, 8 (11). pp. 1599-1611. doi:10.1016/j.molp.2015.06.004 ISSN 0894-0282.
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Official URL: http://dx.doi.org/10.1016/j.molp.2015.06.004
Abstract
Plastidial ω-3 desaturase FAD7 is a major contributor to trienoic fatty acid biosynthesis in the leaves of Arabidopsis plants. However, the precise contribution of the other plastidial ω-3 desaturase, FAD8, is poorly understood. Fatty acid and lipid analysis of several ω-3 desaturase mutants, including two insertion lines of AtFAD7 and AtFAD8, showed that FAD8 partially compensated the disruption of the AtFAD7 gene at 22°C, indicating that FAD8 was active at this growth temperature, contrasting to previous observations that circumscribed the FAD8 activity at low temperatures. Our data revealed that FAD8 had a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied. Differences in the mechanism controlling AtFAD7 and AtFAD8 gene expression at different temperatures were also detected. Confocal microscopy and biochemical analysis of FAD8-YFP over-expressing lines confirmed the chloroplast envelope localization of FAD8. Co-localization experiments suggested that FAD8 and FAD7 might be located in close vicinity in the envelope membrane. FAD8-YFP over-expressing lines showed a specific increase in 18:3 fatty acids at 22°C. Together, these results indicate that the function of both plastidial ω-3 desaturases is coordinated in a non-redundant manner.
Item Type: | Journal Article | ||||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
Journal or Publication Title: | Molecular Plant - Microbe Interactions | ||||||||||
Publisher: | American Phytopathological Society | ||||||||||
ISSN: | 0894-0282 | ||||||||||
Official Date: | October 2015 | ||||||||||
Dates: |
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Volume: | 8 | ||||||||||
Number: | 11 | ||||||||||
Page Range: | pp. 1599-1611 | ||||||||||
DOI: | 10.1016/j.molp.2015.06.004 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Open Access (Creative Commons) |
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