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The deubiquitinating enzyme UBPY is required for Lysosomal Biogenesis and productive autophagy in drosophila
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Jacomin, Anne-Claire, Bescond, Amandine, Soleilhac, Emmanuelle, Gallet, Benoît, Schoehn, Guy, Fauvarque, Marie-Odile and Taillebourg, Emmanuel (2015) The deubiquitinating enzyme UBPY is required for Lysosomal Biogenesis and productive autophagy in drosophila. PLoS One, 10 (11). e0143078. doi:10.1371/journal.pone.0143078
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Official URL: http://dx.doi.org/10.1371/journal.pone.0143078
Abstract
Autophagy is a catabolic process that delivers cytoplasmic components to the lysosomes. Protein modification by ubiquitination is involved in this pathway: it regulates the stability of autophagy regulators such as BECLIN-1 and it also functions as a tag targeting specific substrates to autophagosomes. In order to identify deubiquitinating enzymes (DUBs) involved in autophagy, we have performed a genetic screen in the Drosophila larval fat body. This screen identified Uch-L3, Usp45, Usp12 and Ubpy. In this paper, we show that Ubpy loss of function results in the accumulation of autophagosomes due to a blockade of the autophagy flux. Furthermore, analysis by electron and confocal microscopy of Ubpy-depleted fat body cells revealed altered lysosomal morphology, indicating that Ubpy inactivation affects lysosomal maintenance and/or biogenesis. Lastly, we have shown that shRNA mediated inactivation of UBPY in HeLa cells affects autophagy in a different way: in UBPY-depleted HeLa cells autophagy is deregulated.
| Item Type: | Journal Article | ||||||
|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QL Zoology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||
| Library of Congress Subject Headings (LCSH): | Lysosomes, Life -- Origin, Drosophila | ||||||
| Journal or Publication Title: | PLoS One | ||||||
| Publisher: | Public Library of Science | ||||||
| ISSN: | 1932-6203 | ||||||
| Official Date: | 16 November 2015 | ||||||
| Dates: |
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| Volume: | 10 | ||||||
| Number: | 11 | ||||||
| Article Number: | e0143078 | ||||||
| DOI: | 10.1371/journal.pone.0143078 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access | ||||||
| Funder: | France. Grenoble Partnership for Structural Biology (PSB) | ||||||
| Grant number: | ISBG; UMS 3518 CNRS-CEA-UJF-EMBL, ANR-10-INSB-05-02, ANR-10-LABX-49-01 |
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