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Inferring biomarkers for Mycobacterium avium subsp. paratuberculosis infection and disease progression in cattle using experimental data
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Magombedze, Gesham, Shiri, Tinevimbo, Eda, Shigetoshi and Stabel, Judy R. (2017) Inferring biomarkers for Mycobacterium avium subsp. paratuberculosis infection and disease progression in cattle using experimental data. Scientific Reports, 7 . 44765. doi:10.1038/srep44765 ISSN 2045-2322.
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WRAP-inferring -biomarkers-Mycobacterium-avium subsp. paratuberculosis-Shiri-2017.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3007Kb) | Preview |
Official URL: http://www.nature.com/articles/srep44765
Abstract
Available diagnostic assays for Mycobacterium avium subsp. paratuberculosis (MAP) have poor sensitivities and cannot detect early stages of infection, therefore, there is need to find new diagnostic markers for early infection detection and disease stages. We analyzed longitudinal IFN-γ, ELISA-antibody and fecal shedding experimental sensitivity scores for MAP infection detection and disease progression. We used both statistical methods and dynamic mathematical models to (i) evaluate the empirical assays (ii) infer and explain biological mechanisms that affect the time evolution of the biomarkers, and (iii) predict disease stages of 57 animals that were naturally infected with MAP. This analysis confirms that the fecal test is the best marker for disease progression and illustrates that Th1/Th2 (IFN-γ/ELISA antibodies) assays are important for infection detection, but cannot reliably predict persistent infections. Our results show that the theoretical simulated macrophage-based assay is a potential good diagnostic marker for MAP persistent infections and predictor of disease specific stages. We therefore recommend specifically designed experiments to test the use of a based assay in the diagnosis of MAP infections.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QR Microbiology Q Science > QR Microbiology > QR180 Immunology S Agriculture > SF Animal culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Clinical Trials Unit Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Health Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Mycobacterium avium paratuberculosis, Bacterial diseases, Systems theory, Ruminants -- Diseases | ||||||||
| Journal or Publication Title: | Scientific Reports | ||||||||
| Publisher: | Nature Publishing Group | ||||||||
| ISSN: | 2045-2322 | ||||||||
| Official Date: | 20 March 2017 | ||||||||
| Dates: |
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| Volume: | 7 | ||||||||
| Article Number: | 44765 | ||||||||
| DOI: | 10.1038/srep44765 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Date of first compliant deposit: | 7 April 2017 | ||||||||
| Date of first compliant Open Access: | 10 April 2017 | ||||||||
| Adapted As: |
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