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Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid-state NMR
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Simmons, Thomas J., Mortimer, Jenny C., Bernardinelli, Oigres D., Pöppler, Ann Christin, Brown, Steven P., de Azevedo, Eduardo R, Dupree, Ray and Dupree, Paul (2016) Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid-state NMR. Nature Communications, 7 . 13902 . doi:10.1038/ncomms13902 ISSN 2041-1723.
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Official URL: http://doi.org/10.1038/ncomms13902
Abstract
Exploitation of plant lignocellulosic biomass is hampered by our ignorance of the molecular basis for its properties such as strength and digestibility. Xylan, the most prevalent non-cellulosic polysaccharide, binds to cellulose microfibrils. The nature of this interaction remains unclear, despite its importance. Here we show that the majority of xylan, which forms a threefold helical screw in solution, flattens into a twofold helical screw ribbon to bind intimately to cellulose microfibrils in the cell wall. 13C solid-state magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy, supported by in silico predictions of chemical shifts, shows both two- and threefold screw xylan conformations are present in fresh Arabidopsis stems. The twofold screw xylan is spatially close to cellulose, and has similar rigidity to the cellulose microfibrils, but reverts to the threefold screw conformation in the cellulose-deficient irx3 mutant. The discovery that induced polysaccharide conformation underlies cell wall assembly provides new principles to understand biomass properties.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QK Botany | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Physics | ||||||||
SWORD Depositor: | Library Publications Router | ||||||||
Library of Congress Subject Headings (LCSH): | Xylans, Polysaccharides, Microfibrils, Arabidopsis | ||||||||
Journal or Publication Title: | Nature Communications | ||||||||
Publisher: | Nature Publishing Group | ||||||||
ISSN: | 2041-1723 | ||||||||
Official Date: | 21 December 2016 | ||||||||
Dates: |
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Volume: | 7 | ||||||||
Article Number: | 13902 | ||||||||
DOI: | 10.1038/ncomms13902 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 13 January 2017 | ||||||||
Date of first compliant Open Access: | 13 January 2017 | ||||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Royal Society (Great Britain). Newton International Fellowship (RSNIF), Leverhulme Trust (LT), Engineering and Physical Sciences Research Council (EPSRC), Universty of Warwick, Birmingham Science City, Advantage West Midlands (AWM), European Regional Development Fund (ERDF) | ||||||||
Grant number: | BB/G016240/1 (BBSRC), 159341/2011-6, 206278/2014-4 (CNPq), Contract reference PR140003 (ERDF) | ||||||||
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