AbrB determines the growth phase-dependent transcription of bmrCD. The upper panel shows the transcriptional activity (TAU) of the 5′bmrC-gfp fusion in the parental strain B. subtilis 168 (thick gray line) and in an abrB deletion mutant (thick black line) upon lincomycin induction. The thin lines depict growth of the abrB-proficient (gray) and abrB-deficient (black) strains carrying the 5′bmrC-gfp fusion. Lincomycin was added to the cells during exponential growth as indicated with an arrow. The lower panel shows the transcriptional activity (TAU) of the 5′bmrC-gfp fusion in the parental strain 168 (thick gray line) and the abrB deletion mutant (thick black line) grown in the absence of lincomycin. Note that the patterns of the expression profiles for strain 168 are qualitatively similar to those shown in Figures 2 and 3D, but that the arbitrary TAU are quantitatively different due to the use of plate readers with different detector sensitivity.
