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Mobility measurements probe conformational changes in membrane proteins due to tension
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Morris, Richard G. and Turner, Matthew S. (2015) Mobility measurements probe conformational changes in membrane proteins due to tension. Physical Review Letters, 115 (19). 198101. doi:10.1103/PhysRevLett.115.198101 ISSN 0031-9007.
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Official URL: http://dx.doi.org/10.1103/PhysRevLett.115.198101
Abstract
The function of membrane-embedded proteins such as ion channels depends crucially on their conformation. We demonstrate how conformational changes in asymmetric membrane proteins may be inferred from measurements of their diffusion. Such proteins cause local deformations in the membrane, which induce an extra hydrodynamic drag on the protein. Using membrane tension to control the magnitude of the deformations, and hence the drag, measurements of diffusivity can be used to infer—via an elastic model of the protein—how conformation is changed by tension. Motivated by recent experimental results [Quemeneur et al., Proc. Natl. Acad. Sci. U.S.A. 111, 5083 (2014)], we focus on KvAP, a voltage-gated potassium channel from Aeropyrum pernix. The conformation of KvAP is found to change considerably due to tension, with its “walls,” where the protein meets the membrane, undergoing significant angular strains. The torsional stiffness is determined to be 26.8kBT per radian at room temperature. This has implications for both the structure and the function of such proteins in the environment of a tension-bearing membrane.
Item Type: | Journal Article | ||||||
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Journal or Publication Title: | Physical Review Letters | ||||||
Publisher: | American Physical Society | ||||||
ISSN: | 0031-9007 | ||||||
Official Date: | 6 November 2015 | ||||||
Dates: |
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Volume: | 115 | ||||||
Number: | 19 | ||||||
Article Number: | 198101 | ||||||
DOI: | 10.1103/PhysRevLett.115.198101 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
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