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Chromosome congression is promoted by CENP-Q- and CENP-E-dependent pathways
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Bancroft, James M., Auckland, Philip, Samora, C. P. and McAinsh, Andrew D. (2015) Chromosome congression is promoted by CENP-Q- and CENP-E-dependent pathways. Journal of Cell Science, 128 (1). pp. 171-184. doi:10.1242/jcs.163659 ISSN 0021-9533.
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Official URL: http://dx.doi.org/10.1242/jcs.163659
Abstract
A key step of mitosis is the congression of chromosomes to the spindle equator. Congression is driven by at least two distinct mechanisms: (1) kinetochores slide along the microtubule lattice using the plus-end directed CENP-E motor, and (2) kinetochores biorientating near the pole move to the equator through microtubule depolymerisation-coupled pulling. Here, we show that CENP-Q - a subunit of the CENP-O complex (comprising CENP-O, CENP-P, CENP-Q and CENP-U) that targets polo-like kinase (Plk1) to kinetochores - is also required for the recruitment of CENP-E to kinetochores. We further reveal a CENP-E recruitment-independent role for CENP-Q in depolymerisation-coupled pulling. Both of these functions are abolished by a single point mutation in CENP-Q (S50A) - a residue that is phosphorylated in vivo. Importantly, the S50A mutant does not affect the loading of Plk1 onto kinetochores and leaves the CENP-O complex intact. Thus, the functions of CENP-Q in CENP-E loading and depolymerisation-coupled pulling are independent from its role in Plk1 recruitment and CENP-O complex stabilisation. Taken together, our data provide evidence that phosphoregulation of CENP-Q plays a central function in coordinating chromosome congression mechanisms.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Mitosis, Chromosomes , Spindle (Cell division), Microtubules | ||||||||
| Journal or Publication Title: | Journal of Cell Science | ||||||||
| Publisher: | The Company of Biologists Ltd. | ||||||||
| ISSN: | 0021-9533 | ||||||||
| Official Date: | 1 January 2015 | ||||||||
| Dates: |
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| Volume: | 128 | ||||||||
| Number: | 1 | ||||||||
| Page Range: | pp. 171-184 | ||||||||
| DOI: | 10.1242/jcs.163659 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 15 January 2017 | ||||||||
| Date of first compliant Open Access: | 17 January 2017 | ||||||||
| Funder: | Medical Research Council (Great Britain) (MRC), Fundação para a Ciência e a Tecnologia (FCT), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||
| Grant number: | MR/J003964/1 (MRC), BB/I021353/1 (BBSRC) |
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