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Curvature-induced expulsion of actomyosin bundles during cytokinetic ring contraction
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Huang, Junqi, Chew, Ting Gang, Gu, Ying, Palani, Saravanan, Kamnev, Anton, Martin, Douglas S., Carter, Nicholas J., Cross, R. A., Oliferenko, Snezhana and Balasubramanian, Mohan K. (2016) Curvature-induced expulsion of actomyosin bundles during cytokinetic ring contraction. eLife, 5 . e21383. doi:10.7554/eLife.21383 ISSN 2050-084X.
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Official URL: http://dx.doi.org/10.7554/eLife.21383
Abstract
Many eukaryotes assemble a ring-shaped actomyosin network that contracts to drive cytokinesis. Unlike actomyosin in sarcomeres, which cycles through contraction and relaxation, the cytokinetic ring disassembles during contraction through an unknown mechanism. Here we find in Schizosaccharomyces japonicus and Schizosaccharomyces pombe that, during actomyosin ring contraction, actin filaments associated with actomyosin rings are expelled as micron-scale bundles containing multiple actomyosin ring proteins. Using functional isolated actomyosin rings we show that expulsion of actin bundles does not require continuous presence of cytoplasm. Strikingly, mechanical compression of actomyosin rings results in expulsion of bundles predominantly at regions of high curvature. Our work unprecedentedly reveals that the increased curvature of the ring itself promotes its disassembly. It is likely that such a curvature-induced mechanism may operate in disassembly of other contractile networks.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Protista, Actomyosin, Cytokinesis, Schizosaccharomyces pombe, Cytoplasm | |||||||||||||||
| Journal or Publication Title: | eLife | |||||||||||||||
| Publisher: | eLife Sciences Publications Ltd. | |||||||||||||||
| ISSN: | 2050-084X | |||||||||||||||
| Official Date: | 13 October 2016 | |||||||||||||||
| Dates: |
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| Volume: | 5 | |||||||||||||||
| Article Number: | e21383 | |||||||||||||||
| DOI: | 10.7554/eLife.21383 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
| Date of first compliant deposit: | 25 January 2017 | |||||||||||||||
| Date of first compliant Open Access: | 25 January 2017 | |||||||||||||||
| RIOXX Funder/Project Grant: |
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