Huang, Junqi, Chew, Ting Gang, Gu, Ying, Palani, Saravanan, Kamnev, Anton, Martin, Douglas S., Carter, Nicholas J., Cross, R. A., Oliferenko, Snezhana and Balasubramanian, Mohan K. (2016) Curvature-induced expulsion of actomyosin bundles during cytokinetic ring contraction. eLife, 5 . doi:10.7554/eLife.21383

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Abstract

Many eukaryotes assemble a ring-shaped actomyosin network that contracts to drive cytokinesis. Unlike actomyosin in sarcomeres, which cycles through contraction and relaxation, the cytokinetic ring disassembles during contraction through an unknown mechanism. Here we find in Schizosaccharomyces japonicus and Schizosaccharomyces pombe that, during actomyosin ring contraction, actin filaments associated with actomyosin rings are expelled as micron-scale bundles containing multiple actomyosin ring proteins. Using functional isolated actomyosin rings we show that expulsion of actin bundles does not require continuous presence of cytoplasm. Strikingly, mechanical compression of actomyosin rings results in expulsion of bundles predominantly at regions of high curvature. Our work unprecedentedly reveals that the increased curvature of the ring itself promotes its disassembly. It is likely that such a curvature-induced mechanism may operate in disassembly of other contractile networks.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history Q Science > QR Microbiology
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Protista, Actomyosin, Cytokinesis, Schizosaccharomyces pombe, Cytoplasm
Journal or Publication Title:	eLife
Publisher:	eLife Sciences Publications Ltd.
ISSN:	2050-084X
Official Date:	13 October 2016
Dates:	Date Event 13 October 2016 Published 12 October 2016 Accepted 9 September 2016 Submitted
Date of first compliant deposit:	25 January 2017
Volume:	5
DOI:	10.7554/eLife.21383
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID 103895/Z/14/Z Wellcome Trust http://dx.doi.org/10.13039/100010269 103741/Z/14/Z Wellcome Trust http://dx.doi.org/10.13039/100010269 WT101885MA Wellcome Trust http://dx.doi.org/10.13039/100010269 UNSPECIFIED [RS] Royal Society http://dx.doi.org/10.13039/501100000288
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