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Isolation of Cytokinetic actomyosin rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe
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Huang, Junqi, Mishra, Mithilesh, Palani, Saravanan, Chew, Ting Gang and Balasubramanian, Mohan K. (2016) Isolation of Cytokinetic actomyosin rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe. In: Yeast Cytokinesis : Methods and Protocols. Methods in Molecular Biology, 1369 . Springer New York, pp. 125-136. ISBN 9781493931446
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Official URL: http://dx.doi.org/10.1007/978-1-4939-3145-3_10
Abstract
Cytokinesis is the final stage of cell division, through which cellular constituents of mother cells are partitioned into two daughter cells resulting in the increase in cell number. In animal and fungal cells cytokinesis is mediated by an actomyosin contractile ring, which is attached to the overlying cell membrane. Contraction of this ring after chromosome segregation physically severs the mother cell into two daughters. Here we describe methods for the isolation and partial purification of the actomyosin ring from the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae, which can serve as in vitro systems to facilitate biochemical and ultrastructural analysis of cytokinesis in these genetically tractable model systems.
Item Type: | Book Item | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Series Name: | Methods in Molecular Biology | ||||
Publisher: | Springer New York | ||||
ISBN: | 9781493931446 | ||||
ISSN: | 1064-3745 | ||||
Book Title: | Yeast Cytokinesis : Methods and Protocols | ||||
Official Date: | 2016 | ||||
Dates: |
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Volume: | 1369 | ||||
Page Range: | pp. 125-136 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published |
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