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Quantitative visualization of molecular delivery and uptake at living cells with self-referencing scanning ion conductance microscopy (SICM) – scanning electrochemical microscopy (SECM)
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Page, Ashley M., Kang, Minkyung, Armitstead, Alexander, Perry, David and Unwin, Patrick R. (2017) Quantitative visualization of molecular delivery and uptake at living cells with self-referencing scanning ion conductance microscopy (SICM) – scanning electrochemical microscopy (SECM). Analytical Chemistry, 89 (5). pp. 3021-3028. doi:10.1021/acs.analchem.6b04629 ISSN 0003-2700.
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Official URL: http://dx.doi.org/10.1021/acs.analchem.6b04629
Abstract
A multifunctional dual-channel scanning probe nanopipette that enables simultaneous scanning ion conductance microscopy (SICM) and scanning electrochemical microscopy (SECM) measurements is demonstrated to have powerful new capabilities for spatially mapping the uptake of molecules of interest at living cells. One barrel of the probe is filled with electrolyte and the molecules of interest and is open to the bulk solution for both topographical feedback and local delivery to a target interface, while a solid carbon electrode in the other barrel measures the local concentration and flux of the delivered molecules. This setup allows differentiation in molecular uptake rate across several regions of single cells with individual measurements at nanoscale resolution. Further, operating in a ‘hopping mode’, where the probe is translated towards the interface (cell) at each point allows self-referencing to be employed, in which the carbon electrode response is calibrated at each and every pixel for comparison to the bulk measurement. This is particularly important for measurements in living systems where an electrode response may change over time. Finite element method (FEM) modeling places the technique on a quantitative footing to allow the response of the carbon electrode and local delivery rates to be quantified. The technique is extremely versatile, with the local delivery of molecules highly tuneable via control of the SICM bias to promote or restrict migration from the pipette orifice. It is expected to have myriad applications from drug delivery to screening catalysts.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history R Medicine > RS Pharmacy and materia medica |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Library of Congress Subject Headings (LCSH): | Scanning electrochemical microscopy, Scanning probe microscopy , Electrochemistry, Drug delivery systems | ||||||||
Journal or Publication Title: | Analytical Chemistry | ||||||||
Publisher: | American Chemical Society | ||||||||
ISSN: | 0003-2700 | ||||||||
Official Date: | 7 March 2017 | ||||||||
Dates: |
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Volume: | 89 | ||||||||
Number: | 5 | ||||||||
Page Range: | pp. 3021-3028 | ||||||||
DOI: | 10.1021/acs.analchem.6b04629 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 25 January 2017 | ||||||||
Date of first compliant Open Access: | 24 January 2018 | ||||||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), University of Warwick. Molecular Organisation and Assembly in Cells (MOAC), University of Warwick Chancellor’s Interational Scholarship, European Research Council (ERC), Leverhulme Trust (LT) | ||||||||
Grant number: | EP/F500378/1, (EPSRC), Grant ERC-2009-AdG 247143- QUANTIF (ERC) |
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