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Structural mechanism for bacterial oxidation of oceanic trimethylamine into trimethylamine N -oxide
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Li, Chun-Yang, Chen, Xiu-Lan, Zhang, Dian, Wang, Peng, Sheng, Qi, Peng, Ming, Xie, Bin-Bin, Qin, Qi-Long, Li, Ping-Yi, Zhang, Xi-Ying, Su, Hai-Nan, Song, Xiao-Yan, Shi, Mei, Zhou, Bai-Cheng, Xun, Lu-Ying, Chen, Yin and Zhang, Yu-Zhong (2017) Structural mechanism for bacterial oxidation of oceanic trimethylamine into trimethylamine N -oxide. Molecular Microbiology, 103 (6). pp. 992-1003. doi:10.1111/mmi.13605 ISSN 0950-382X.
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Official URL: http://dx.doi.org/10.1111/mmi.13605
Abstract
Trimethylamine (TMA) and trimethylamine N-oxide (TMAO) are widespread in the ocean and are important nitrogen source for bacteria. TMA monooxygenase (Tmm), a bacterial flavin-containing monooxygenase (FMO), is found widespread in marine bacteria and is responsible for converting TMA to TMAO. However, the molecular mechanism of TMA oxygenation by Tmm has not been explained. Here, we determined the crystal structures of two reaction intermediates of a marine bacterial Tmm (RnTmm) and elucidated the catalytic mechanism of TMA oxidation by RnTmm. The catalytic process of Tmm consists of a reductive half-reaction and an oxidative half-reaction. In the reductive half-reaction, FAD is reduced and a C4a-hydroperoxyflavin intermediate forms. In the oxidative half-reaction, this intermediate attracts TMA through electronic interactions. After TMA binding, NADP+ bends and interacts with D317, shutting off the entrance to create a protected micro-environment for catalysis and exposing C4a-hydroperoxyflavin to TMA for oxidation. Sequence analysis suggests that the proposed catalytic mechanism is common for bacterial Tmms. These findings reveal the catalytic process of TMA oxidation by marine bacterial Tmm and first show that NADP+ undergoes a conformational change in the oxidative half-reaction of FMOs.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Marine bacteria, Oxidation, Nitrogen, Catalysis | ||||||||
Journal or Publication Title: | Molecular Microbiology | ||||||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||
ISSN: | 0950-382X | ||||||||
Official Date: | March 2017 | ||||||||
Dates: |
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Volume: | 103 | ||||||||
Number: | 6 | ||||||||
Page Range: | pp. 992-1003 | ||||||||
DOI: | 10.1111/mmi.13605 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 26 January 2017 | ||||||||
Date of first compliant Open Access: | 26 January 2017 | ||||||||
Funder: | Guo jia zi ran ke xue ji jin wei yuan hui (China) [National Natural Science Foundation of China] (NSFC), Zhongguo ke xue yuan [Chinese Academy of Sciences] (CAS), GuĂłjiÄ gÄo jĂŹshĂč yĂĄnjiĆ« fÄzhÇn jĂŹhuĂ (China) [National High Technology Research Development Program] (NHTRDP), ZhĆnghuĂĄ rĂ©nmĂn gĂČnghĂ©guĂł rĂ©nlĂŹ zÄ«yuĂĄn hĂ© shĂšhuĂŹ bÇozhĂ ng bĂč (China) [Ministry of Human Resources and Social Security of the People 's Republic of China] (MOHRSS) | ||||||||
Grant number: | 31290231, 31630012 (NSFC), TS20090803 (CAS), 2012AA092105 (NHTRDP), BX201600095 (MOHRSS) |
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