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Identification of dimethylamine monooxygenase in marine bacteria reveals a metabolic bottleneck in the methylated amine degradation pathway
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Lidbury, Ian, Mausz, Michaela A., Scanlan, David J. and Chen, Yin (2017) Identification of dimethylamine monooxygenase in marine bacteria reveals a metabolic bottleneck in the methylated amine degradation pathway. ISME Journal, 11 . pp. 1592-1601. doi:10.1038/ismej.2017.31
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Official URL: https://doi.org/10.1038/ismej.2017.31
Abstract
Methylated amines (MAs) are ubiquitous in the marine environment and their subsequent flux into the atmosphere can result in the formation of aerosols and ultimately cloud condensation nuclei. Therefore, these compounds have a potentially important role in climate regulation. Using Ruegeria pomeroyi as a model, we identified the genes encoding dimethylamine (DMA) monooxygenase (dmmABC) and demonstrate that this enzyme degrades DMA to monomethylamine (MMA). Although only dmmABC are required for enzyme activity in recombinant Escherichia coli, we found that an additional gene, dmmD, was required for the growth of R. pomeroyi on MAs. The dmmDABC genes are absent from the genomes of multiple marine bacteria, including all representatives of the cosmopolitan SAR11 clade. Consequently, the abundance of dmmDABC in marine metagenomes was substantially lower than the genes required for other metabolic steps of the MA degradation pathway. Thus, there is a genetic and potential metabolic bottleneck in the marine MA degradation pathway. Our data provide an explanation for the observation that DMA-derived secondary organic aerosols (SOAs) are among the most abundant SOAs detected in fine marine particles over the North and Tropical Atlantic Ocean.
| Item Type: | Journal Article | ||||||||
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| Subjects: | G Geography. Anthropology. Recreation > G Geography (General) Q Science > QR Microbiology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Marine bacteria -- Genetic aspects, Atlantic Ocean | ||||||||
| Journal or Publication Title: | ISME Journal | ||||||||
| Publisher: | Nature Publishing Group | ||||||||
| ISSN: | 1751-7362 | ||||||||
| Official Date: | July 2017 | ||||||||
| Dates: |
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| Volume: | 11 | ||||||||
| Page Range: | pp. 1592-1601 | ||||||||
| DOI: | 10.1038/ismej.2017.31 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Funder: | Natural Environment Research Council (Great Britain) (NERC) | ||||||||
| Grant number: | Grant NE/M002233/1 | ||||||||
| Related URLs: |
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