Nuclear factor-kappa B does not mediate the inhibitory effects of dexamethasone on granulocyte-macrophage colony-stimulating factor expression
UNSPECIFIED. (2004) Nuclear factor-kappa B does not mediate the inhibitory effects of dexamethasone on granulocyte-macrophage colony-stimulating factor expression. IMMUNOLOGY, 111 (4). pp. 430-434. ISSN 0019-2805Full text not available from this repository.
Official URL: http://dx.doi.org/10.1111/j.1365-2567.2004.01833.x
Human granulocyte-macrophage colony-stimulating factor (GM-CSF) reporter constructs containing up to 3.3 kb of upstream promoter sequence were transiently transfected into both Jurkat and HUT78 human T-cell lines. In Jurkat cells, stimulation with phorbol 12-myristate 13-acetate (PMA) plus phytohemaglutinin (PHA) produced robust increases in reporter activity, whereas HUT78 cells showed low levels of reporter induction attributable to constitutive nuclear factor (NF)-kappaB activity. Following mutation of either the proximal NF-kappaB site (-85/-76) or the activator protein1 (AP-1) motif within the conserved lymphokine element 0 (CLE0) site (-54/-31), reporter activity was markedly reduced in both cell lines. Despite this dependence on NF-kappaB and CLE0/AP-1, GM-CSF reporter activity was unaffected by dexamethasone in either cell line. Similarly, an NF-kappaB-dependent reporter was also not repressed by dexamethasone, yet GM-CSF release from HUT78 T cells was inhibited. These data therefore confirm a critical role for both NF-kappaB and CLE0 sites in GM-CSF promoter activation and indicate that NF-kappaB may not mediate glucocorticoid-dependent repression of GM-CSF in these cells.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology > QR180 Immunology|
|Journal or Publication Title:||IMMUNOLOGY|
|Publisher:||BLACKWELL PUBLISHING LTD|
|Official Date:||April 2004|
|Number of Pages:||5|
|Page Range:||pp. 430-434|
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