Zhu, Chongyu, Jinxin, Zhao, Kempe, Kristian, Wilson, Paul, Wang, Jiping , Velkov, Tony , Li, Jian , Davis, Thomas P., Whittaker, Michael R. and Haddleton, David M. (2017) Data for A hydrogel based localized release of colistin for antimicrobial treatment of burn wound infection. [Dataset]

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Abstract

There is an urgent unmet medical need for new treatments for wound and burn infections caused by multidrug-resistant (MDR) Gram-negative ‘superbugs’, especially the problematic Pseudomonas aeruginosa. In this work we report the incorporation of colistin, a potent lipopeptide into a self-healable hydrogel (via dynamic imine bond formation) following the chemical reaction between the amine groups present in glycol chitosan and an aldehyde modified poly (ethylene glycol) (PEG). The storage module (G’) of the colistin-loaded hydrogel ranged from 1.3 kPa to 5.3 kPa by varying the amount of the cross-linker and colistin loading providing different options for topical wound healing. The majority of the colistin is released from the hydrogel within 24 h and remains active as demonstrated by both antibacterial in vitro disk diffusion and time-kill assays. Moreover and pleasingly, the colistin-loaded hydrogel performed almost equally well as native colistin against both the colistin-sensitive and also colistin-resistant P. aeruginosa strain in the in vivo animal 'burn' infection model despite exhibiting a slower killing profile in vitro. Based on this antibiotic performance along with the biodegradability of the product, we believe the colistin-loaded hydrogel to be a potential localized wound-healing formulation to treat burn wounds against microbial infection.

Item Type:	Dataset
Subjects:	Q Science > QD Chemistry Q Science > QP Physiology R Medicine > RC Internal medicine
Divisions:	Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH):	Colloids, Burns and scalds -- Treatment, Wounds and injuries -- Treatment, Anti-infective agents
Journal or Publication Title:	Macromolecular Bioscience
Publisher:	Department of Chemistry, University of Warwick
ISSN:	1616-5187
Official Date:	24 February 2017
Dates:	Date Event 24 February 2017 Accepted 24 February 2017 Available
Date of first compliant deposit:	17 August 2016
DOI:	10.1002/mabi.201600320
Status:	Not Peer Reviewed
Access rights to Published version:	Open Access
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID Wolfson Merit Award Royal Society http://dx.doi.org/10.13039/501100000288 UNSPECIFIED [EPSRC] Engineering and Physical Sciences Research Council http://dx.doi.org/10.13039/501100000266 UNSPECIFIED Leverhulme Trust http://dx.doi.org/10.13039/501100000275 CE140100036 Australian Research Council http://dx.doi.org/10.13039/501100000923
Related URLs:	Publisher Related item in WRAP
Contributors:	Contribution Name Contributor ID Depositor Zhu, Chongyu 60011

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