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A computational method for three-dimensional reconstruction of the microarchitecture of myometrial smooth muscle from histological sections
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Lutton, E. Josiah, Lammers, Wim J. E. P., James, Sean G., Berg, Hugo van den and Blanks, Andrew M. (2017) A computational method for three-dimensional reconstruction of the microarchitecture of myometrial smooth muscle from histological sections. PLoS One, 12 (3). e0173404. doi:10.1371/journal.pone.0173404 ISSN 1932-6203.
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Official URL: https://doi.org/10.1371/journal.pone.0173404
Abstract
Background: The fibrous structure of the myometrium has previously been characterised at high resolutions in small tissue samples (< 100 mm3) and at low resolutions (∼ 500 µm per voxel edge) in whole-organ reconstructions. However, no high-resolution visualisation of the myometrium at the organ level has previously been attained. Methods and Results: We have developed a technique to reconstruct the whole myometrium from serial histological slides, at a resolution of approximately 50 µm per voxel edge. Reconstructions of samples taken from human and rat uteri are presented here, along with histological verification of the reconstructions and detailed investigation of the fibrous structure of these uteri, using a range of tools specifically developed for this analysis. These reconstruction techniques enable the high-resolution rendering of global structure previously observed at lower resolution. Moreover, structures observed previously in small portions of the myometrium can be observed in the context of the whole organ. The reconstructions are in direct correspondence with the original histological slides, which allows the inspection of the anatomical context of any features identified in the three-dimensional reconstructions. Conclusions and Significance: The methods presented here have been used to generate a faithful representation of myometrial smooth muscle at a resolution of ∼ 50 µm per voxel edge. Characterisation of the smooth muscle structure of the myometrium by means of this technique revealed a detailed view of previously identified global structures in addition to a global view of PLOS 1/50 the microarchitecture. A suite of visualisation tools allows researchers to interrogate the histological microarchitecture. These methods will be applicable to other smooth muscle tissues to analyse fibrous microarchitecture.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QM Human anatomy | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Science > Mathematics Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Myometrium , Histology, Imaging systems in medicine | ||||||
Journal or Publication Title: | PLoS One | ||||||
Publisher: | Public Library of Science | ||||||
ISSN: | 1932-6203 | ||||||
Official Date: | 16 March 2017 | ||||||
Dates: |
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Volume: | 12 | ||||||
Number: | 3 | ||||||
Article Number: | e0173404 | ||||||
DOI: | 10.1371/journal.pone.0173404 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 23 February 2017 | ||||||
Date of first compliant Open Access: | 31 July 2017 | ||||||
Funder: | Medical Research Council (Great Britain) (MRC) | ||||||
Grant number: | Grant number G0900208-2 | ||||||
RIOXX Funder/Project Grant: |
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