Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Methylamine as a nitrogen source for microorganisms from a coastal marine environment

Tools
- Tools
+ Tools

Taubert, Martin, Grob, Carolina, Howat, Alexandra M., Burns, Oliver J., Pratscher, Jennifer, Jehmlich, Nico, von Bergen, Martin, Richnow, Hans H., Chen, Yin and Murrell, J. C. (J. Colin) (2017) Methylamine as a nitrogen source for microorganisms from a coastal marine environment. Environmental Microbiology, 19 (6). pp. 2246-2257. doi:10.1111/1462-2920.13709

[img]
Preview
PDF
WRAP-methylamine-nitrogen-source-microorganisms-Chen-2017.pdf - Accepted Version - Requires a PDF viewer.

Download (907Kb) | Preview
Official URL: https://dx.doi.org/10.1111/1462-2920.13709

Request Changes to record.

Abstract

Nitrogen is a key limiting resource for biomass production in the marine environment. Methylated amines, released from the degradation of osmolytes, could provide a nitrogen source for marine microbes. Thus far, studies in aquatic habitats on the utilization of methylamine, the simplest methylated amine, have mainly focussed on the fate of the carbon from this compound. Various groups of methylotrophs, microorganisms that can grow on one-carbon compounds, use methylamine as a carbon source. Non-methylotrophic microorganisms may also utilize methylamine as a nitrogen source, but little is known about their diversity, especially in the marine environment. In this proof-of-concept study, stable isotope probing (SIP) was used to identify microorganisms from a coastal environment that assimilate nitrogen from methylamine. SIP experiments using 15N methylamine combined with metagenomics and metaproteomics facilitated identification of active methylamine-utilizing Alpha- and Gammaproteobacteria. The draft genomes of two methylamine utilizers were obtained and their metabolism with respect to methylamine was examined. Both bacteria identified in these SIP experiments used the γ-glutamyl-methylamide pathway, found in both methylotrophs and non-methylotrophs, to metabolize methylamine. The utilization of 15N methylamine also led to the release of 15N ammonium that was used as nitrogen source by other microorganisms not directly using methylamine.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Nitrogen cycle, Marine biology, Microorganisms, Methylotrophic microorganisms, Amines, Metagenomics, Proteobacteria
Journal or Publication Title: Environmental Microbiology
Publisher: Blackwell
ISSN: 1462-2920
Official Date: June 2017
Dates:
DateEvent
June 2017Published
29 March 2017Available
20 February 2017Accepted
Volume: 19
Number: 6
Page Range: pp. 2246-2257
DOI: 10.1111/1462-2920.13709
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIEDEuropean Regional Development Fundhttp://dx.doi.org/10.13039/501100008530
UNSPECIFIEDHelmholtz Associationhttp://dx.doi.org/10.13039/501100009318
GBMF3303Gordon and Betty Moore Foundationhttp://dx.doi.org/10.13039/100000936
UNSPECIFIEDEarth and Life Systems Alliancehttp://dx.doi.org/10.13039/501100006406

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us