Footitt, Steven, Ölcer-Footitt, Hülya, Hambidge, Angela J. and Finch-Savage, William E. (2017) A laboratory simulation of Arabidopsis seed dormancy cycling provides new insight into its regulation by clock genes and the dormancy-related genes DOG1 , MFT , CIPK23 and PHYA. Plant, Cell & Environment, 40 (8). pp. 1474-1486. doi:10.1111/pce.12940

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Abstract

Environmental signals drive seed dormancy cycling in the soil to synchronise germination with the optimal time of year; a process essential for species fitness and survival. Previous correlation of transcription profiles in exhumed seeds with annual environmental signals revealed the coordination of dormancy regulating mechanisms with the soil environment. Here, we developed a rapid and robust laboratory dormancy cycling simulation. The utility of this simulation was tested in two ways. Firstly using mutants in known dormancy-related genes (DELAY OF GERMINATION 1 (DOG1), MOTHER OF FLOWERING TIME (MFT), CBL-INTERACTING PROTEIN KINASE 23 (CIPK23) and PHYTOCHROME A (PHYA)). Secondly, using further mutants we test the hypothesis that components of the circadian clock are involved in coordination of the annual seed dormancy cycle. The rate of dormancy induction and relief differed in all lines tested. In the mutants, dog1-2 and mft2, dormancy induction was reduced but not absent. DOG1 is not absolutely required for dormancy. In cipk23 and phyA dormancy induction was accelerated. Involvement of the clock in dormancy cycling was clear when mutants in the morning and evening loops of the clock were compared. Dormancy induction was faster when the morning loop was compromised and delayed when the evening loop was compromised.

Item Type:	Journal Article
Subjects:	Q Science > QK Botany
Divisions:	Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH):	Arabidopsis -- Seeds -- Dormancy, Arabidopsis -- Life cycles, Germination, Plant life cycles, Circadian rhythms
Journal or Publication Title:	Plant, Cell & Environment
Publisher:	Wiley-Blackwell Publishing Ltd.
ISSN:	0140-7791
Official Date:	August 2017
Dates:	Date Event August 2017 Published 27 February 2017 Available 19 February 2017 Accepted 1 December 2016 Submitted
Date of first compliant deposit:	28 February 2017
Volume:	40
Number:	8
Page Range:	pp. 1474-1486
DOI:	10.1111/pce.12940
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC)
Grant number:	BB/I022201/1

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