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Current advances in molecular methods for detection of nitrite-dependent anaerobic methane oxidizing bacteria in natural environments
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Chen, Jing, Dick, Richard, Lin, Jih-Gaw and Gu, Ji-Dong (2016) Current advances in molecular methods for detection of nitrite-dependent anaerobic methane oxidizing bacteria in natural environments. Applied Microbiology and Biotechnology, 100 (23). pp. 9845-9860. doi:10.1007/s00253-016-7853-5 ISSN 0175-7598.
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Official URL: http://dx.doi.org/10.1007/s00253-016-7853-5
Abstract
Nitrite-dependent anaerobic methane oxidation (n-damo) process uniquely links microbial nitrogen and carbon cycles. Research on n-damo bacteria progresses quickly with experimental evidences through enrichment cultures. Polymerase chain reaction (PCR)-based methods for detecting them in various natural ecosystems and engineered systems play a very important role in the discovery of their distribution, abundance, and biodiversity in the ecosystems. Important characteristics of n-damo enrichments were obtained and their key significance in microbial nitrogen and carbon cycles was investigated. The molecular methods currently used in detecting n-damo bacteria were comprehensively reviewed and discussed for their strengths and limitations in applications with a wide range of samples. The pmoA gene-based PCR primers for n-damo bacterial detection were evaluated and, in particular, several incorrectly stated PCR primer nucleotide sequences in the published papers were also pointed out to allow correct applications of the PCR primers in current and future investigations. Furthermore, this review also offers the future perspectives of n-damo bacteria based on current information and methods available for a better acquisition of new knowledge about this group of bacteria.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QH Natural history | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
Library of Congress Subject Headings (LCSH): | Nitrogen cycle , Carbon cycle (Biogeochemistry) | ||||||||||
Journal or Publication Title: | Applied Microbiology and Biotechnology | ||||||||||
Publisher: | Springer | ||||||||||
ISSN: | 0175-7598 | ||||||||||
Official Date: | December 2016 | ||||||||||
Dates: |
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Volume: | 100 | ||||||||||
Number: | 23 | ||||||||||
Page Range: | pp. 9845-9860 | ||||||||||
DOI: | 10.1007/s00253-016-7853-5 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 3 March 2017 | ||||||||||
Date of first compliant Open Access: | 20 October 2017 | ||||||||||
Funder: | University of Hong Kong (HKU). PhD Fellowship , Research Grants Council (Hong Kong, China) | ||||||||||
Grant number: | Grant No. 701913 (Research Grants Council (Hong Kong, China)) |
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