Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Cellular reactions to long-term volatile organic compound (VOC) exposures

Tools
- Tools
+ Tools

Gostner, Johanna M., Zeisler, Johannes, Alam, Mohammad T., Gruber, Peter, Fuchs, Dietmar, Becker, Kathrin, Neubert, Kerstin, Kleinhappl, Markus, Martini, Stefan and Überall, Florian (2016) Cellular reactions to long-term volatile organic compound (VOC) exposures. Scientific Reports, 6 . 37842.

[img]
Preview
PDF
WRAP_Gostner et al 2016.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution 4.0.

Download (2558Kb) | Preview
Official URL: http://dx.doi.org/10.1038/srep37842

Request Changes to record.

Abstract

Investigations of cellular processes initiated by volatile organic compounds (VOCs) are limited when modelling realistic long-term exposure scenarios at low concentrations. Exposure to indoor VOCs is associated with a range of adverse effects, but data on molecular changes at regulatory threshold limits are lacking. Activity analysis of VOC in vitro can be a valuable complement to inhalation toxicological evaluations. We developed an exposure platform that generates a stable VOC atmosphere and allows the exposure of cells for longer periods. Using formaldehyde as a model analyte, air-liquid interface cultured A549 lung epithelial cells were exposed to critical concentrations of 0.1 and 0.5 ppm for 3 days. Owing to the lack of known exposure biomarkers, we applied a genome-wide transcriptional analysis to investigate cellular responses at these sublethal concentrations. We demonstrate a minor overlap of differentially expressed transcripts for both treatment concentrations, which can be further analyzed for their use as exposure biomarkers. Moreover, distinct expression patterns emerge for 0.1 and 0.5 ppm formaldehyde exposure, which is reflected in significant enrichment of distinct biological processes. More specifically, metabolism of specific compound classes, lipid biosynthesis and lung-associated functions are affected by lower exposure levels and processes affecting proliferation and apoptosis dominate the higher exposure levels.

Item Type: Journal Article
Subjects: T Technology > TD Environmental technology. Sanitary engineering
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Volatile organic compounds -- Health aspects
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
Official Date: 1 December 2016
Dates:
DateEvent
1 December 2016Published
31 October 2016Accepted
21 April 2016Submitted
Volume: 6
Article Number: 37842
Status: Peer Reviewed
Publication Status: Published
Funder: Fonds zur Förderung der Wissenschaftlichen Forschung (Austria) (FWF), Österreichische Forschungsförderungsgesellschaft (FFG)
Grant number: MUI-START project 2014-05-023 (FWF), FFG834169 (FFG)

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us