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The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase
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Yurkovich, Marie E., Jenkins, Robert, Sun, Yuhui, Tosin, Manuela and Leadlay, P. F. (2017) The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase. Chemical Communications, 53 (13). pp. 2182-2185. doi:10.1039/C6CC09934C ISSN 1359-7345.
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WRAP-Tosin-thiolactomycin-iterative-polyketide-synthase-Yurkovich-2017.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (1082Kb) | Preview |
Official URL: http://dx.doi.org/10.1039/C6CC09934C
Abstract
Following the in vivo investigation of thiotetronate assembly in Lentzea sp. and in S. thiolactonus NRRL 15439 (Havemann et al., Chem. Commun., 2017, DOI: 10.1039/c6cc09933e), the minimal set of genes required for thiolactomycin production was determined through heterologous expression and the mechanism for polyketide assembly was established in vitro through incubation of recombinant TlmB with its substrates in the presence of either nonhydrolysable or hydrolysable chemical probes. The results presented here constitute unequivocal evidence of enzymatic processing by an unusual iterative polyketide synthase.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QP Physiology Q Science > QR Microbiology R Medicine > RM Therapeutics. Pharmacology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||
Library of Congress Subject Headings (LCSH): | Antibiotics, Drug resistance in microorganisms, Polyketides -- Synthesis, Antitubercular agents, Antimalarials, Trypanosomiasis | ||||||||||
Journal or Publication Title: | Chemical Communications | ||||||||||
Publisher: | Royal Society of Chemistry | ||||||||||
ISSN: | 1359-7345 | ||||||||||
Official Date: | 2017 | ||||||||||
Dates: |
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Volume: | 53 | ||||||||||
Number: | 13 | ||||||||||
Page Range: | pp. 2182-2185 | ||||||||||
DOI: | 10.1039/C6CC09934C | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||
Date of first compliant deposit: | 13 March 2017 | ||||||||||
Date of first compliant Open Access: | 13 March 2017 | ||||||||||
Funder: | Natural Sciences and Engineering Research Council of Canada (NSERC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Engineering and Physical Sciences Research Council (EPSRC) | ||||||||||
Grant number: | BB/J007250/1 (BBSRC) |
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