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Design and implementation of a biomolecular concentration tracker
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Hsiao, Victoria, Emmanuel de los Santos, L. C., Whitaker, Weston R., Dueber, John E. and Murray, Richard M. (2015) Design and implementation of a biomolecular concentration tracker. ACS Synthetic Biology, 4 (2). pp. 150-161. doi:10.1021/sb500024b ISSN 2161-5063.
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Official URL: http://dx.doi.org/10.1021/sb500024b
Abstract
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Cells, Synthetic biology, Biotechnology | ||||||||
| Journal or Publication Title: | ACS Synthetic Biology | ||||||||
| Publisher: | American Chemical Society | ||||||||
| ISSN: | 2161-5063 | ||||||||
| Official Date: | 20 February 2015 | ||||||||
| Dates: |
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| Volume: | 4 | ||||||||
| Number: | 2 | ||||||||
| Page Range: | pp. 150-161 | ||||||||
| DOI: | 10.1021/sb500024b | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 5 April 2017 | ||||||||
| Date of first compliant Open Access: | 10 April 2017 | ||||||||
| Funder: | National Defense Science and Engineering Graduate (NDSEG), Benjamin M. Rosen Bioengineering Center, Gordon and Betty Moore Foundation, Institute for Collaborative Biotechnologies (ICB) | ||||||||
| Grant number: | GM07616, GBMF2809, W911NF-09-0001 |
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