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Design and implementation of a biomolecular concentration tracker

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Hsiao, Victoria, Emmanuel de los Santos, L. C., Whitaker, Weston R., Dueber, John E. and Murray, Richard M. (2015) Design and implementation of a biomolecular concentration tracker. ACS Synthetic Biology, 4 (2). pp. 150-161. doi:10.1021/sb500024b ISSN 2161-5063.

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Official URL: http://dx.doi.org/10.1021/sb500024b

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Abstract

As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Cells, Synthetic biology, Biotechnology
Journal or Publication Title: ACS Synthetic Biology
Publisher: American Chemical Society
ISSN: 2161-5063
Official Date: 20 February 2015
Dates:
DateEvent
20 February 2015Published
6 May 2015Available
6 May 2015Accepted
Volume: 4
Number: 2
Page Range: pp. 150-161
DOI: 10.1021/sb500024b
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 5 April 2017
Date of first compliant Open Access: 10 April 2017
Funder: National Defense Science and Engineering Graduate (NDSEG), Benjamin M. Rosen Bioengineering Center, Gordon and Betty Moore Foundation, Institute for Collaborative Biotechnologies (ICB)
Grant number: GM07616, GBMF2809, W911NF-09-0001

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