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Evidence for the formation of ScbR/ScbR2 heterodimers and identification of one of the regulatory targets in Streptomyces coelicolor
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Li, Xiao, Wang, Juan, Shi, Mingxin, Wang, Weishan, Corre, Christophe and Yang, Keqian (2017) Evidence for the formation of ScbR/ScbR2 heterodimers and identification of one of the regulatory targets in Streptomyces coelicolor. Applied Microbiology and Biotechnology, 101 (13). pp. 5333-5340. ISSN 0175-7598.
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WRAP-evidence-formation-ScbR-ScbR2-heterodimers-Corre-2017.pdf - Accepted Version - Requires a PDF viewer. Download (1239Kb) | Preview |
Official URL: https://doi.org/10.1007/s00253-017-8275-8
Abstract
The homologous transcriptional regulators ScbR and ScbR2 have previously been identified as γ-butyrolactone (GBL) and antibiotic receptors, respectively. They regulate diverse physiological processes in Streptomyces coelicolor in response to GBL and antibiotic signals. In this study, ScbR and ScbR2 proteins were shown to interact using a bacterial two-hybrid system where adenylate cyclase activity was reconstituted in Escherichia coli BH101. These ScbR/ScbR2 interactions in S. coelicolor were then demonstrated by co-immunoprecipitation. The ScbR/ScbR2 heterodimer was shown to co-exist with their ScbR and ScbR2 respective homodimers. When potential operator targets in S. coelicolor were investigated, the heterodimer was found to bind in the promoter region of sco5158, which however was not a target for ScbR or ScbR2 homodimers. These results revelaed a new mechanism
25 of regulation by ScbR and ScbR2 in S. coelicolor.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Streptomyces coelicolor, Protein-protein interactions. | ||||||||
Journal or Publication Title: | Applied Microbiology and Biotechnology | ||||||||
Publisher: | Springer | ||||||||
ISSN: | 0175-7598 | ||||||||
Official Date: | July 2017 | ||||||||
Dates: |
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Volume: | 101 | ||||||||
Number: | 13 | ||||||||
Page Range: | pp. 5333-5340 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 25 April 2017 | ||||||||
Date of first compliant Open Access: | 24 April 2018 | ||||||||
Funder: | Guo jia zi ran ke xue ji jin wei yuan hui (China) [National Natural Science Foundation of China] (NSFC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||
Grant number: | 31570031, BB/M022765/1 |
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