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Assays to monitor aggrephagy in Drosophila brain
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Jacomin, Anne-Claire and Nezis, Ioannis P. (2017) Assays to monitor aggrephagy in Drosophila brain. In: Turksen, K., (ed.) Autophagy in Differentiation and Tissue Maintenance. Methods in Molecular Biology, 1854 . New York, NY: Humana Press, pp. 147-157. ISBN 9781493987474
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Official URL: https://doi.org/10.1007/7651_2018_157
Abstract
Accumulation of ubiquitinated protein aggregates is a hallmark of most ageingrelated neurodegenerative disorders. Autophagy has been found to be involved in the selective clearance of these protein aggregates, and this process is called aggrephagy. Here we provide two protocols for the investigation of protein aggregation and their removal by autophagy using western blotting and immunofluorescence techniques in Drosophila brain. Investigating the role of aggrephagy at the cellular and organismal level is important for the development of therapeutic interventions against ageing-related diseases.
| Item Type: | Book Item | |||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
| Library of Congress Subject Headings (LCSH): | Cell death, Proteins, Drosophila -- Physiological aspects | |||||||||
| Series Name: | Methods in Molecular Biology | |||||||||
| Journal or Publication Title: | Methods in Molecular Biology | |||||||||
| Publisher: | Humana Press | |||||||||
| Place of Publication: | New York, NY | |||||||||
| ISBN: | 9781493987474 | |||||||||
| ISSN: | 1064-3745 | |||||||||
| Book Title: | Autophagy in Differentiation and Tissue Maintenance | |||||||||
| Editor: | Turksen, K. | |||||||||
| Official Date: | 5 June 2017 | |||||||||
| Dates: |
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| Volume: | 1854 | |||||||||
| Page Range: | pp. 147-157 | |||||||||
| DOI: | 10.1007/7651_2018_157 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Restricted or Subscription Access | |||||||||
| Date of first compliant deposit: | 28 April 2017 | |||||||||
| Date of first compliant Open Access: | 5 June 2019 | |||||||||
| RIOXX Funder/Project Grant: |
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